https://scholars.lib.ntu.edu.tw/handle/123456789/572755
標題: | Discovery and characterization of diterpene synthases in Chamaecyparis formosensis Matsum. which participated in an unprecedented diterpenoid biosynthesis route in conifer | 作者: | Ma L.-T Wang C.-H Hon C.-Y Lee Y.-R FANG-HUA CHU |
關鍵字: | diterpenoid; ent-kaurene synthetase A; plant protein; terpene synthase; transferase; Chamaecyparis; enzymology; Escherichia coli; gene expression profiling; metabolism; molecular cloning; nuclear magnetic resonance spectroscopy; transgenic organism; Alkyl and Aryl Transferases; Chamaecyparis; Cloning, Molecular; Diterpenes; Escherichia coli; Gene Expression Profiling; Magnetic Resonance Spectroscopy; Metabolic Networks and Pathways; Organisms, Genetically Modified; Plant Proteins | 公開日期: | 2021 | 卷: | 304 | 來源出版物: | Plant Science | 摘要: | Chamaecyparis formosensis Matsum. is an endemic and precious coniferous species of Taiwan, and is known for a high abundance of specialized metabolites, which contributes to the excellent timber durability. Several terpenoids were identified and isolated from C. formosensis wood and needles, and exhibit anti-fungal and anti-bacterial bioactivities, which may participate in plant defense against pathogens. In various identified compounds, not only cadinene and ferruginol, were identified in C. formosensis extracts but also unique diterpenoids, which include pisferal, totarol, and derivates of isoabienol. To understand the biosynthesis of these specific diterpenoids, we conducted a series of functional characterization of the C. formosensis diterpene synthases (CfdiTPSs), which participate in skeleton formation and differentiation of diterpenes. In this study, we identified eight diTPSs from C. formosensis transcriptome, and they all contain either class I or class II motif, which indicates they are all monofunctional enzymes. These candidates consist of three class II diTPSs and five class I diTPSs, and after conducting in vivo and in vitro assays, class II diTPS CfCPS1 was characterized as a (+)-copalyl diphosphate synthase ((+)-CPS), and class I diTPSs CfKSL1 could further convert (+)-copalyl diphosphate ((+)-CPP) to levopimaradiene. Meanwhile, CfKSL1 also accepted labda-13-en-8-ol diphosphate (LPP) as substrate and formed monoyl oxide. Another class I diTPS, CfKSL4, exhibits a strong enzymatic ability of isoabienol synthase, which is firstly reported in conifer. This finding provides potential participants in the biosynthesis of unique diterpenoids, and with this knowledge, we can further expand our understanding of diterpenoid metabolism in Cupressaceae and their potential role in plant defense. ? 2020 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098173178&doi=10.1016%2fj.plantsci.2020.110790&partnerID=40&md5=c13d47f771b0be04a99cde893912f1b0 https://scholars.lib.ntu.edu.tw/handle/123456789/572755 |
ISSN: | 1689452 | DOI: | 10.1016/j.plantsci.2020.110790 |
顯示於: | 森林環境暨資源學系 |
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