https://scholars.lib.ntu.edu.tw/handle/123456789/572795
標題: | Microfluidic colorimetric analysis system for sodium benzoate detection in foods | 作者: | Ko C.-H Liu C.-C Chen K.-H FUU SHEU Fu L.-M Chen S.-J. |
關鍵字: | Acetone; Benzoic acid; Beverages; Cellular telephones; Chemical reactions; CMOS integrated circuits; Color; Colorimetric analysis; Colorimetry; Deterioration; Microfluidics; Microprocessor chips; MOS devices; Oxide semiconductors; Sodium; Sodium hydroxide; Analysis equipments; Analysis softwares; Beverage products; Calibration curves; CMOS (complementary metal oxide semiconductor); Colorimetric reaction; Concentration detections; Determination coefficients; Spoilage; acetic acid; acetone; acetonitrile; acetophenone; benzoic acid; citric acid; dehydroacetic acid; food preservative; metal oxide; methanol; nitro derivative; sodium chloride; sorbate potassium; benzoic acid; food additive; Article; beverage; calcination temperature; calibration; carbonated beverage; color; colorimetry; comparative study; controlled study; food; food analysis; food composition; heating; high performance liquid chromatography; limit of detection; microchip analysis; microfluidics; pickled vegetable; reaction analysis; reaction time; tofu; ultraviolet spectrophotometry; colorimetry; food analysis; human; microfluidic analysis; procedures; standard; Beverages; Chromatography, High Pressure Liquid; Colorimetry; Food Additives; Food Analysis; Humans; Microfluidic Analytical Techniques; Reference Standards; Sodium Benzoate | 公開日期: | 2021 | 卷: | 345 | 來源出版物: | Food Chemistry | 摘要: | Sodium benzoate (SBA) is a widely-used additive for preventing food spoilage and deterioration and extending the shelf life. However, the concentration of SBA must be controlled under safe regulations to avoid damaging human health. Accordingly, this study proposes a microfluidic colorimetric analysis (MCA) system composing of a wax-printed paper-microchip and a self-made smart analysis equipment for the concentration detection of SBA in common foods and beverages. In the presented method, the distilled SBA sample is mixed with NaOH to obtain a nitro compound and the compound is then dripped onto the reaction area of the paper-microchip, which is embedded with two layers of reagents (namely acetophenone and acetone). The paper-microchip is heated at 120 °C for 20 min to cause a colorimetric reaction and the reaction image is then obtained through a CMOS (complementary metal oxide semiconductor) device and transmitted to a cell-phone over a WiFi connection. Finally, use the self-developed RGB analysis software installed on the cell-phone to obtain the SBA concentration. A calibration curve is constructed using SBA samples with known concentrations ranging from 50 ppm (0.35 mM) to 5000 ppm (35 mM). It is shown that the R + G + B value (Y) of the reaction image and SBA concentration (X) are related via Y = ?0.034 X +737.40, with a determination coefficient of R2 = 0.9970. By measuring the SBA concentration of 15 commercially available food and beverage products, the actual feasibility of the current MCA system can be demonstrated. The results show that the difference from the measurement results obtained using the macroscale HPLC method does not exceed 6.0%. Overall, the current system provides a reliable and low-cost technique for quantifying the SBA concentration in food and drink products. ? 2020 Elsevier Ltd |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097582805&doi=10.1016%2fj.foodchem.2020.128773&partnerID=40&md5=3c9141f83cb2ea01c64ffbc218119281 https://scholars.lib.ntu.edu.tw/handle/123456789/572795 |
ISSN: | 3088146 | DOI: | 10.1016/j.foodchem.2020.128773 | SDG/關鍵字: | Acetone; Benzoic acid; Beverages; Cellular telephones; Chemical reactions; CMOS integrated circuits; Color; Colorimetric analysis; Colorimetry; Deterioration; Microfluidics; Microprocessor chips; MOS devices; Oxide semiconductors; Sodium; Sodium hydroxide |
顯示於: | 園藝暨景觀學系 |
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