Portable paper-micro well device composed of agglomerated nano-hematite clusters in enzyme-hydrogel composite for beta glucan detection using smartphone
Journal
Sensors and Actuators, B: Chemical
Journal Volume
339
Date Issued
2021
Author(s)
Bagal-Kestwal D.R
Abstract
In present study, we have harnessed the irreplaceable merits of nano-biocomposite biosensing for microfluidic devices preparation. A microfluidic device-prototype (μPADs) with coroneted immobilized enzymes on hematite nanoparticle (αFe2O3NPs) was designed for direct detection of cancer marker β-glucan. Reconfigurable open-smartphone software applications (App) with visual detection and smartphone application can further ease the collection, calculation and display of sensor data on the screen for the biomarker analyte. The bienzymatic-microfluidic analytical devices (Enz-μPADs) with micro-well test-zone were coated with β-glucanase, glucose oxidase, and fenugreek hydrogel stabilized-αFe2O3NPs. Distinct blue color change of as-prepared Enz-μPADs can also be observed by naked eyes and quantified by smartphone App with high accuracy. The smartphone images represented by the saturation parameter of hue–saturation–value (HSV) color space, enables an easy readout of the enzymatic-TMB reaction for β-glucan detection. The linear calibration graph was obtained in the range of 1.0–15 ng mL?1 for standard β-glucan with 0.5 ng mL?1 limit of detection (3 Sb). The practical applicability of Enz-μPADs was tested successfully for anti-cancer dietary-supplements with β-glucan claims. Thus, as-prepared Enz-μPADs with Smartphone-App readout has notable features such as fast colorimetric visual response, low-cost, feasible device bulk production and multiplexity with high specificity and sensitivity. ? 2021 Elsevier B.V.
Subjects
Agglomeration; Analytic equipment; Application programs; Color; Colorimetry; Diseases; Fluidic devices; Glucose; Glucose oxidase; Glucose sensors; Hematite; Hydrogels; Microfluidics; Smartphones; Agglomerated hematite nanoparticle/cluster; Enzymatic microfluidic paper analytical device; Glucans; Micro wells; Micro-fluidic devices; Smart phones; Smart-phone applications; Smartphone software application; Β-glucan biosensor; Β-glucanase; Nanoparticles
SDGs
Other Subjects
Agglomeration; Analytic equipment; Application programs; Color; Colorimetry; Diseases; Fluidic devices; Glucose; Glucose oxidase; Glucose sensors; Hematite; Hydrogels; Microfluidics; Smartphones; Agglomerated hematite nanoparticle/cluster; Enzymatic micro
Type
journal article