https://scholars.lib.ntu.edu.tw/handle/123456789/572878
標題: | Portable paper-micro well device composed of agglomerated nano-hematite clusters in enzyme-hydrogel composite for beta glucan detection using smartphone | 作者: | Bagal-Kestwal D.R BEEN-HUANG CHIANG |
關鍵字: | Agglomeration; Analytic equipment; Application programs; Color; Colorimetry; Diseases; Fluidic devices; Glucose; Glucose oxidase; Glucose sensors; Hematite; Hydrogels; Microfluidics; Smartphones; Agglomerated hematite nanoparticle/cluster; Enzymatic microfluidic paper analytical device; Glucans; Micro wells; Micro-fluidic devices; Smart phones; Smart-phone applications; Smartphone software application; Β-glucan biosensor; Β-glucanase; Nanoparticles | 公開日期: | 2021 | 卷: | 339 | 來源出版物: | Sensors and Actuators, B: Chemical | 摘要: | In present study, we have harnessed the irreplaceable merits of nano-biocomposite biosensing for microfluidic devices preparation. A microfluidic device-prototype (μPADs) with coroneted immobilized enzymes on hematite nanoparticle (αFe2O3NPs) was designed for direct detection of cancer marker β-glucan. Reconfigurable open-smartphone software applications (App) with visual detection and smartphone application can further ease the collection, calculation and display of sensor data on the screen for the biomarker analyte. The bienzymatic-microfluidic analytical devices (Enz-μPADs) with micro-well test-zone were coated with β-glucanase, glucose oxidase, and fenugreek hydrogel stabilized-αFe2O3NPs. Distinct blue color change of as-prepared Enz-μPADs can also be observed by naked eyes and quantified by smartphone App with high accuracy. The smartphone images represented by the saturation parameter of hue–saturation–value (HSV) color space, enables an easy readout of the enzymatic-TMB reaction for β-glucan detection. The linear calibration graph was obtained in the range of 1.0–15 ng mL?1 for standard β-glucan with 0.5 ng mL?1 limit of detection (3 Sb). The practical applicability of Enz-μPADs was tested successfully for anti-cancer dietary-supplements with β-glucan claims. Thus, as-prepared Enz-μPADs with Smartphone-App readout has notable features such as fast colorimetric visual response, low-cost, feasible device bulk production and multiplexity with high specificity and sensitivity. ? 2021 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103987506&doi=10.1016%2fj.snb.2021.129836&partnerID=40&md5=35134441d48d7bc153fffe303394d759 https://scholars.lib.ntu.edu.tw/handle/123456789/572878 |
ISSN: | 9254005 | DOI: | 10.1016/j.snb.2021.129836 | SDG/關鍵字: | Agglomeration; Analytic equipment; Application programs; Color; Colorimetry; Diseases; Fluidic devices; Glucose; Glucose oxidase; Glucose sensors; Hematite; Hydrogels; Microfluidics; Smartphones; Agglomerated hematite nanoparticle/cluster; Enzymatic micro |
顯示於: | 食品科技研究所 |
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