https://scholars.lib.ntu.edu.tw/handle/123456789/572954
標題: | An automatic microfluidic DNA microarray platform for SNP detection using a DNA intercalating dye and graphene oxide | 作者: | Huang S.-H Chang Y.-S Chang K.-W MONG-HSUN TSAI Huang N.-T. |
關鍵字: | DNA; Dyes; Graphene oxide; Microarrays; Microfluidics; Nucleotides; Clinical treatments; DNA hybridization; DNA micro-array; Genetic screening; Intercalating dye; Microfluidic platforms; Perfect matches; Single nucleotide polymorphisms; Graphene | 公開日期: | 2020 | 起(迄)頁: | 1237-1238 | 來源出版物: | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 | 摘要: | We developed a fully automatic microfluidic platform integrating DNA hybridization processes to reduce the hybridization time and eliminate potential manipulation errors. By using a novel DNA hybridization protocol of adding SYBR green I (SGI) and graphene oxide (GO) sequentially, the signal difference between perfect and non-perfect matches can be enhanced. Our platform can achieve single nucleotide polymorphisms (SNPs) detection in 2.5 hours and has the potential to provide accessible genetic screening for timely clinical treatments. ? 17CBMS-0001. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079688217&partnerID=40&md5=c597bb744e6fb18fa6bc070f70f848a1 https://scholars.lib.ntu.edu.tw/handle/123456789/572954 |
顯示於: | 生物科技研究所 |
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