Publication: An automatic microfluidic DNA microarray platform for SNP detection using a DNA intercalating dye and graphene oxide
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Date
2020
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Abstract
We developed a fully automatic microfluidic platform integrating DNA hybridization processes to reduce the hybridization time and eliminate potential manipulation errors. By using a novel DNA hybridization protocol of adding SYBR green I (SGI) and graphene oxide (GO) sequentially, the signal difference between perfect and non-perfect matches can be enhanced. Our platform can achieve single nucleotide polymorphisms (SNPs) detection in 2.5 hours and has the potential to provide accessible genetic screening for timely clinical treatments. ? 17CBMS-0001.
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Keywords
DNA; Dyes; Graphene oxide; Microarrays; Microfluidics; Nucleotides; Clinical treatments; DNA hybridization; DNA micro-array; Genetic screening; Intercalating dye; Microfluidic platforms; Perfect matches; Single nucleotide polymorphisms; Graphene