https://scholars.lib.ntu.edu.tw/handle/123456789/573199
標題: | Bushmeat species identification: Recombinase polymerase amplification (rpa) combined with lateral flow (lf) strip for identification of formosan reeves’ muntjac (muntiacus reevesi micrurus) | 作者: | Hsu Y.-H WEI-CHENG YANG Chan K.-W. |
關鍵字: | cyanocobalamin; cytochrome b; recombinase; sesame seed oil; aquaculture; Article; breast cancer; controlled study; cost effectiveness analysis; DNA extraction; gene amplification; India; limit of detection; mastoidectomy; Micrurus; moisture; muntjac; nonhuman; operation duration; ostrich; Pholidota (animal); pig; polymerase chain reaction; public health; resuscitation; species identification; temperature; wild meat; wildlife | 公開日期: | 2021 | 卷: | 11 | 期: | 2 | 起(迄)頁: | 1-11 | 來源出版物: | Animals | 摘要: | The identification of animal species of meat in meat products is of great concern for various reasons, such as public health, religious beliefs, food allergies, legal perspectives, and bushmeat control. In this study, we developed a new technique to identify Formosan Reeves’ muntjac in meat using recombinase polymerase amplification (RPA) in combination with a lateral flow (LF) strip. The DNA extracted from a piece of Formosan Reeves’ muntjac meat was amplified by a pair of specific primers based on its mitochondrial cytochrome b gene for 10 min at a constant temperature ranging from 30 to 45?C using RPA. Using the specific probe added to the RPA reaction system, the amplified products were visualized on the LF strip within 5 min. The total operating time from quick DNA extraction to visualizing the result was approximately 30 min. The RPA-LF system we designed was efficient when using boiled, pan-fried, roasted, stir-fried, or stewed samples. The advantages of simple operation, speediness, and cost-effectiveness make our RPA-LF method a promising molecular detection tool for meat species identification of either raw or variously cooked Formosan Reeves’ muntjac meat. It is also possible to apply this method to identify the meat of other wildlife sources. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100459777&doi=10.3390%2fani11020426&partnerID=40&md5=4a6fc6d70a1b29214c5900f9b7d23882 https://scholars.lib.ntu.edu.tw/handle/123456789/573199 |
ISSN: | 20762615 | DOI: | 10.3390/ani11020426 | SDG/關鍵字: | cyanocobalamin; cytochrome b; recombinase; sesame seed oil; aquaculture; Article; breast cancer; controlled study; cost effectiveness analysis; DNA extraction; gene amplification; India; limit of detection; mastoidectomy; Micrurus; moisture; muntjac; nonhuman; operation duration; ostrich; Pholidota (animal); pig; polymerase chain reaction; public health; resuscitation; species identification; temperature; wild meat; wildlife |
顯示於: | 獸醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。