https://scholars.lib.ntu.edu.tw/handle/123456789/573322
標題: | Bioelectronic control of a microbial community using surface-assembled electrogenetic cells to route signals | 作者: | Terrell J.L HSUAN-CHEN WU et al. |
關鍵字: | Gene expression; Molecules; Signal transduction; Biological functions; Biological signals; Electronic feedback; Electronic signals; Microbial communities; Molecular communication; Quorum sensing molecules; Therapeutic peptides; Electrodes; Article; bacterial cell; bacterial strain; coculture; controlled study; electrogenetics; feedback system; gene expression; gene technology; genetic engineering; information processing; microbial community; nonhuman; oxidation reduction reaction; plankton; protein secretion; quorum sensing; signal transduction | 公開日期: | 2021 | 卷: | 16 | 期: | 6 | 起(迄)頁: | 688-697 | 來源出版物: | Nature Nanotechnology | 摘要: | We developed a bioelectronic communication system that is enabled by a redox signal transduction modality to exchange information between a living cell-embedded bioelectronics interface and an engineered microbial network. A naturally communicating three-member microbial network is ‘plugged into’ an external electronic system that interrogates and controls biological function in real time. First, electrode-generated redox molecules are programmed to activate gene expression in an engineered population of electrode-attached bacterial cells, effectively creating a living transducer electrode. These cells interpret and translate electronic signals and then transmit this information biologically by producing quorum sensing molecules that are, in turn, interpreted by a planktonic coculture. The propagated molecular communication drives expression and secretion of a therapeutic peptide from one strain and simultaneously enables direct electronic feedback from the second strain, thus enabling real-time electronic verification of biological signal propagation. Overall, we show how this multifunctional bioelectronic platform, termed a BioLAN, reliably facilitates on-demand bioelectronic communication and concurrently performs programmed tasks. ? 2021, The Author(s), under exclusive licence to Springer Nature Limited. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103386323&doi=10.1038%2fs41565-021-00878-4&partnerID=40&md5=1dd44d47baeb325ee6eaef6e7680113c https://scholars.lib.ntu.edu.tw/handle/123456789/573322 |
ISSN: | 17483387 | DOI: | 10.1038/s41565-021-00878-4 |
顯示於: | 生化科技學系 |
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