https://scholars.lib.ntu.edu.tw/handle/123456789/573325
標題: | A disposable electrochemical sensor designed to estimate glycated hemoglobin (HbA1c) level in whole blood | 作者: | Thiruppathi M Lee J.-F Chen C.C Ho J.-A.A. JA-AN ANNIE HO |
關鍵字: | Blood; Electrochemical sensors; Hemoglobin; High performance liquid chromatography; Ketones; Multiwalled carbon nanotubes (MWCN); Proteins; Chemically modified electrode; Concentration ranges; ELectrochemical methods; Glycated hemoglobin (HbA1c); Glycated hemoglobins; High performance liquid chromatographic methods; Optimized conditions; Screen-printed carbon electrodes; Electrochemical electrodes | 公開日期: | 2021 | 卷: | 329 | 來源出版物: | Sensors and Actuators, B: Chemical | 摘要: | The level of glycated hemoglobin percentage (HbA1c %), referring to the concentration ratio of HbA1c to total hemoglobin (Hb), has been used to accurately determine stage-wise diabetes for a long time period. Measurement of HbA1c alone may result in an error for monitoring glycemia in patients with diabetes. Addressing this challenge, we report an electrochemical method that senses both Hb and HbA1c using two chemically modified electrodes (SPCE/CNT-Nf@Hb-Nf and SPCE*/AQBA-HbA1c). For the determination of Hb, MultiWalled Carbon Nanotubes (MWCNT) with Nafion (Nf) were fabricated on a Screen Printed Carbon Electrode (SPCE), and the resulting modified electrode (SPCE/CNT-Nf@Hb-Nf) provided a redox signal for whole blood/Hb standard samples for electroactive Fe2+/Fe3+ that exist in heme proteins. A voltammetric sensor for HbA1c was developed using anthraquinone boronic acid (AQBA) by modifying a pre-anodized, screen-printed carbon electrode (SPCE*). The resulting SPCE*/AQBA electrode was able to complex with applied HbA1c through specific boronic acid–diol recognition. The binding of AQBA to HbA1c decreased its redox peak current, which led to the determination of HbA1c. Under optimized conditions, the sensor displays a linear response of 9.4–16.4 g/dL Hb and 31.2?500 nM HbA1c concentration range, with detection limit (S/N = 3) of 4.2 nM for HbA1c. In addition, the percentage concentration of HbA1c was successfully determined in whole blood samples collected clinically. The results were also compared with those obtained using the High-Performance Liquid Chromatographic (HPLC) method for validation and the correlation value of 0.95 indicates that this sensor holds promise as a potential alternative method to analyze HbA1c clinically. ? 2020 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096835484&doi=10.1016%2fj.snb.2020.129119&partnerID=40&md5=fb5956065208767c0445f7b9e6a22cad https://scholars.lib.ntu.edu.tw/handle/123456789/573325 |
ISSN: | 9254005 | DOI: | 10.1016/j.snb.2020.129119 | SDG/關鍵字: | Blood; Electrochemical sensors; Hemoglobin; High performance liquid chromatography; Ketones; Multiwalled carbon nanotubes (MWCN); Proteins; Chemically modified electrode; Concentration ranges; ELectrochemical methods; Glycated hemoglobin (HbA1c); Glycated |
顯示於: | 生化科技學系 |
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