https://scholars.lib.ntu.edu.tw/handle/123456789/573335
標題: | Construction of intracellular asymmetry and asymmetric division in Escherichia coli | 作者: | Lin, Da-Wei Liu, Yang Lee, Yue-Qi Yang, Po-Jiun Ho, Chia-Tse Hong, Jui-Chung Hsiao, Jye-Chian Liao, Der-Chien Liang, An-Jou Hung, Tzu-Chiao Chen, Yu-Chuan Tu, Hsiung-Lin Hsu, Chao-Ping HSIAO-CHUN HUANG |
關鍵字: | ampicillin; chloramphenicol; oligomer; RNA polymerase; bacterial protein; cell cycle protein; chromosome partition proteins, bacterial; DivIVA protein, bacteria; biology; cell component; coliform bacterium; differentiation; enzyme; enzyme activity; genetic analysis; microbial activity; protein; Article; asymmetric cell division; Bacillus subtilis; bacterial cell; bacterial genetics; Caulobacter vibrioides; cell differentiation; controlled study; cytology; diffusion; Escherichia coli; gene construct; nonhuman; protein synthesis; cell polarity; cytology; Escherichia coli; gene expression regulation; genetics; intracellular space; metabolism; Bacillus subtilis; Caulobacter vibrioides; Escherichia coli; Asymmetric Cell Division; Bacillus subtilis; Bacterial Proteins; Caulobacter crescentus; Cell Cycle Proteins; Cell Differentiation; Cell Polarity; Escherichia coli; Gene Expression Regulation, Bacterial; Intracellular Space | 公開日期: | 2021 | 卷: | 12 | 期: | 1 | 來源出版物: | Nature Communications | 摘要: | The design principle of establishing an intracellular protein gradient for asymmetric cell division is a long-standing fundamental question. While the major molecular players and their interactions have been elucidated via genetic approaches, the diversity and redundancy of natural systems complicate the extraction of critical underlying features. Here, we take a synthetic cell biology approach to construct intracellular asymmetry and asymmetric division in Escherichia coli, in which division is normally symmetric. We demonstrate that the oligomeric PopZ from Caulobacter crescentus can serve as a robust polarized scaffold to functionalize RNA polymerase. Furthermore, by using another oligomeric pole-targeting DivIVA from Bacillus subtilis, the newly synthesized protein can be constrained to further establish intracellular asymmetry, leading to asymmetric division and differentiation. Our findings suggest that the coupled oligomerization and restriction in diffusion may be a strategy for generating a spatial gradient for asymmetric cell division. ? 2021, The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101015960&doi=10.1038%2fs41467-021-21135-1&partnerID=40&md5=31303a7923740baf71d2a13164377281 https://scholars.lib.ntu.edu.tw/handle/123456789/573335 |
ISSN: | 20411723 | DOI: | 10.1038/s41467-021-21135-1 |
顯示於: | 分子與細胞生物學研究所 |
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