https://scholars.lib.ntu.edu.tw/handle/123456789/575477
標題: | Diagnosis of bladder cancer using telomerase activity in voided urine | 作者: | Cheng C.-W. SHIH-CHIEH CHUEH Chern H.-D. |
關鍵字: | Benign prostatic hyperplasia; Bladder cancer; Non-radioactive assay; Telomerase activity; Voided urine | 公開日期: | 2000 | 卷: | 99 | 期: | 12 | 起(迄)頁: | 920-925 | 來源出版物: | Journal of the Formosan Medical Association | 摘要: | Background and purpose: Telomerase is an essential enzyme for cellular immortality and tumorigenesis. Reactivation of telomerase is associated with many primary cancers. We evaluated the accuracy of a modified immunodiagnostic technique based on the telomeric repeat amplification protocol (TRAP) assay, by semi-quantitative measurement of telomerase activity in exfoliated urothelial cells in voided urine from patients with bladder cancer. Methods: Telomerase activity was assayed in centrifuged urine cell pellets from 17 bladder cancer patients and from 32 patients with benign bladder diseases. Each specimen was collected from a 50-mL sample of single voided urine obtained before surgery, and telomerase activity was detected using a telomerase polymerase chain reaction and enzyme-linked immunosorbent assay (PCR-ELISA) protocol. Results of pathologic study, urine cytologic examination, and urine telomerase activity were determined independently. Results: The cut-off value for relative telomerase activity was set at 0.059, which provided an optimal diagnostic accuracy of 88% (n = 49). At this cut-off value, the sensitivity and specificity for urine telomerase in bladder cancer were 82% (n = 17) and 91% (n = 32), respectively. Telomerase activity was found in 11 low-grade tumors and six high-grade tumors, whereas negative results for telomerase activity were found in urothelial cells of patients with inguinal hernia, urinary stones, acute urinary tract infection, or chronic cystitis. Only five cytology samples from the same patients were positive for bladder cancer. The difference in these two detection rates was significant (p = 0.002). Conclusion: The results of this study indicate that the measurement of telomerase activity from voided urine using our modified semi-quantitative PCR-ELISA technique may help provide earlier diagnosis of bladder cancer and earlier postoperative indication of recurrence. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034533914&partnerID=40&md5=d5d37d2176f1eb9a0cdb104ea936f932 https://scholars.lib.ntu.edu.tw/handle/123456789/575477 |
ISSN: | 0929-6646 | SDG/關鍵字: | telomerase; adult; aged; article; bladder cancer; cancer diagnosis; carcinogenesis; cell immortalization; clinical article; cystitis; cytology; diagnostic accuracy; diagnostic value; early diagnosis; enzyme activity; enzyme linked immunosorbent assay; female; human; inguinal hernia; male; micturition; polymerase chain reaction; prostate hypertrophy; quantitative assay; serodiagnosis; urinary tract infection; urolithiasis; Adult; Aged; Aged, 80 and over; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Middle Aged; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Telomerase; Tumor Markers, Biological; Urinary Bladder Neoplasms; Urine; Urothelium |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。