https://scholars.lib.ntu.edu.tw/handle/123456789/575707
標題: | Remodeling nanoDESI Platform with Ion Mobility Spectrometry to Expand Protein Coverage in Cancerous Tissue | 作者: | Chen C.-L Kuo T.-H Chung H.-H Huang P Lin L.-E CHENG-CHIH HSU |
關鍵字: | Desorption; Electrospray ionization; Image enhancement; Ion mobility spectrometers; Mapping; Mass spectrometry; Proteins; Ambient ionizations; Biological samples; Broad application; Desorption electrospray ionization mass spectrometries; Ion mobility spectrometry; Isobaric interferences; Microscopic image; Spatial resolution; Tissue | 公開日期: | 2021 | 卷: | 32 | 期: | 3 | 起(迄)頁: | 653-660 | 來源出版物: | Journal of the American Society for Mass Spectrometry | 摘要: | Nanospray desorption electrospray ionization mass spectrometry is an ambient ionization technique that is capable of mapping proteins in tissue sections. However, high-abundant molecules or isobaric interference in biological samples hampers its broad applications in probing low-abundant proteins. To address this challenge, herein we demonstrated an integrated module that coupled pneumatic-assisted nanospray desorption electrospray ionization mass spectrometry with high-field asymmetric ion mobility spectrometry. Using this module to analyze mouse brain sections, the protein coverage was significantly increased. This improvement allowed the mapping of low-abundant proteins in tissue sections with a 5 μm spatial resolution enabled by computationally assisted fusion with optical microscopic images. Moreover, the module was successfully applied to characterize melanoma in skin tissues based on the enhanced protein profiles. The results suggested that this integrating module will be potentially applied to discover novel proteins in cancers. ? |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100661182&doi=10.1021%2fjasms.0c00354&partnerID=40&md5=f0d8139414e89951922844bce9cfced1 https://scholars.lib.ntu.edu.tw/handle/123456789/575707 |
ISSN: | 10440305 | DOI: | 10.1021/jasms.0c00354 | SDG/關鍵字: | Desorption; Electrospray ionization; Image enhancement; Ion mobility spectrometers; Mapping; Mass spectrometry; Proteins; Ambient ionizations; Biological samples; Broad application; Desorption electrospray ionization mass spectrometries; Ion mobility spec |
顯示於: | 化學系 |
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