https://scholars.lib.ntu.edu.tw/handle/123456789/575879
標題: | Labeling and Characterization of Phenol-Containing Glycopeptides Using Chemoselective Probes with Isotope Tags | 作者: | Wang T.-S.A Wu R.-Y Hong Y Wang Z.-C Li T.-L Shie J.-J TSUNG-SHING WANG CHENG-CHIH HSU |
公開日期: | 2021 | 來源出版物: | ChemBioChem | 摘要: | Secondary metabolites are structurally diverse natural products (NPs) and have been widely used for medical applications. Developing new tools to enrich NPs can be a promising solution to isolate novel NPs from the native and complex samples. Here, we developed native and deuterated chemoselective labeling probes to target phenol-containing glycopeptides by the ene-type labeling used in proteomic research. The clickable azido-linker was included for further biotin functionalization to facilitate the enrichment of labeled substrates. Afterward, our chemoselective method, in conjunction with LC-MS and MSn analysis, was demonstrated in bacterial cultures. A vancomycin-related phenol-containing glycopeptide was labeled and characterized by our labeling strategy, showing its potential in glycopeptide discovery in complex environments. ? 2021 Wiley-VCH GmbH |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105485767&doi=10.1002%2fcbic.202100169&partnerID=40&md5=6eeed075c1702fa03d548cb72df71061 https://scholars.lib.ntu.edu.tw/handle/123456789/575879 |
ISSN: | 14394227 | DOI: | 10.1002/cbic.202100169 |
顯示於: | 化學系 |
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