Croceicoccus bisphenolivorans sp. Nov., a bisphenol a-degrading bacterium isolated from seawater
Journal
International Journal of Systematic and Evolutionary Microbiology
Journal Volume
71
Journal Issue
2
Pages
1-8
Date Issued
2021
Author(s)
Abstract
A bisphenol A-degrading bacterium, designated as strain H4T, was isolated from surface seawater, which was sampled from the Jiulong River estuary in southeast PR China. Strain H4T is Gram-stain-negative, aerobic, short rod-shaped, lacking bacteriochlorophyll a, motile with multifibrillar stalklike fascicle structures and capable of degrading bisphenol A. Growth of strain H4T was observed at 24–45 °C (optimum, 32 °C), at pH 5.5–9 (optimum, pH 7.0) and in 0–7% NaCl (optimum, 2%; w/v). The 16S rRNA gene sequence of strain H4T showed highest similarity to Croceicoccus pelagius Ery9T (98.7%), Croceicoccus sediminis (98.3%), Croceicoccus naphthovorans PQ-2T (98.1%) and Croceicoccus ponticola GM-16T (97.6%), followed by Croceicoccus marinus E4A9T (96.7%) and Croceicoccus mobilis Ery22T (96.0%). Phylogenetic analysis revealed that strain H4T fell within a clade comprising the type strains of Croceicoccus species and formed a phyletic line with them that was distinct from other members of the family Erythrobacteraceae. The sole respiratory quinone was quinone 10 (Q-10). The predominant fatty acids (>>5% of the total fatty acids) of strain H4T were summed feature 8 (C18:1 ω6c and/or C18:1 ω7c), summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C17:1 ω6c and C14:0 2-OH. The genomic DNA G+C content was 62.8 mol%. In the polar lipid profile, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two sphingoglycolipids and three unknown lipids were the major compounds. Based on the genotypic and phenotypic data, strain H4T represents a novel species of the genus Croceicoccus, for which the name Croceicoccus bisphenolivorans sp. nov. is proposed. The type strain is H4T (=DSM 102182T=MCCC1 K02301T). ? 2021 The Authors.
Subjects
4,4' isopropylidenediphenol; bacteriochlorophyll a; fatty acid; genomic DNA; phosphatidylcholine; phosphatidylethanolamine; phosphatidylglycerol; quinone derivative; RNA 16S; sea water; sodium chloride; 4,4' isopropylidenediphenol; bacterial DNA; benzhydryl derivative; fatty acid; phenol derivative; phospholipid; RNA 16S; sea water; ubidecarenone; ubiquinone; Article; bacterial genome; bacterial growth; bacterium isolation; China; cladistics; controlled study; Croceicoccus bisphenolivorans; Croceicoccus marinus; Croceicoccus mobilis; Croceicoccus naphthovorans; Croceicoccus pelagius; Croceicoccus ponticola; Croceicoccus sediminis; DNA base composition; estuary; gene sequence; Gram negative bacterium; Gram staining; lipid fingerprinting; new species; nonhuman; nucleotide sequence; phylogeny; priority journal; sequence analysis; type strain; Alphaproteobacteria; bacterium identification; chemistry; classification; DNA sequence; genetics; isolation and purification; metabolism; microbiology; phylogeny; Alphaproteobacteria; Bacterial Typing Techniques; Base Composition; Benzhydryl Compounds; China; DNA, Bacterial; Estuaries; Fatty Acids; Phenols; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Ubiquinone
Type
journal article