Library preparation for next-generation sequencing using a multiplex microfluidic chip
Journal
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Journal Volume
4
Pages
2355-2357
Date Issued
2018
Author(s)
Abstract
The emergence of next-generation sequencing (NGS) has propelled genomic analysis into a new era. However, the bottleneck is the library preparation which, for most cases, is labor-intensive and operator-dependent. In this study, a centrifugal-based microfluidic design enables on-chip fragmentation, end repair, A-addition, and ligation. Key features include multiplex processing, substantial reduction in reagent usage, low (?250 pg) input DNA quantity and reliable fluidic control. With simple to operate and low-cost microfluidic chip, the design demonstrated the potential for automated handling for multiplex library preparation for various applications. Copyright ? (2018) by Chemical and Biological Microsystems Society. All rights reserved.
Subjects
Centrifugation; Fluidic devices; Automated handling; Centrifugal microfluidics; Genomic analysis; Low sample input; Microfluidic chip; Next-generation sequencing; Sample preparation; Substantial reduction; Microfluidics
Type
conference paper