https://scholars.lib.ntu.edu.tw/handle/123456789/577057
標題: | Lateral flow immunoassay with stacking effect for the high sensitive Staphylococcus aureus detection | 作者: | Wang H.-P Tsai T.-T Huang T.-H CHIEN-FU CHEN |
關鍵字: | Bacteria; Binding energy; Chemical detection; Electrophoresis; Proteins; Binding interaction; Detection limits; Detection sensitivity; Lateral flow immunoassay; Polyacrylamide gel electrophoresis; Protein A; Stacking effect; Staphylococcus aureus | 公開日期: | 2018 | 卷: | 2 | 起(迄)頁: | 1057-1059 | 來源出版物: | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 | 摘要: | In this paper, we introduce an innovative “stacking pad” for an extended binding interaction of antigen and antibodies to enhance the detection sensitivity. The concept is adapted from a stacking gel of polyacrylamide gel electrophoresis (PAGE) which allows for proteins to stack in a concentrated area and expend the reaction time. We also examined various materials as the stacking pad to push the detection limit of the initial colorimetric signal. In order to verify the feasibility of the proposed platform, Protein A, a specific product of Staphylococcus aureus was chosen as a targeted biomarker. As a result, Protein A can be accurately visible at the 1 ng/mL detection limit in 20 min. Copyright ? (2018) by Chemical and Biological Microsystems Society. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079843881&partnerID=40&md5=34ffd171f7b349efc02ad73dc1908514 https://scholars.lib.ntu.edu.tw/handle/123456789/577057 |
顯示於: | 應用力學研究所 |
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