https://scholars.lib.ntu.edu.tw/handle/123456789/577165
標題: | An electroactive alginate hydrogel nanocomposite reinforced by functionalized graphite nanofilaments for neural tissue engineering | 作者: | Homaeigohar S Tsai T.-Y Young T.-H Yang H.J Ji Y.-R. Young, Tai-Horng |
關鍵字: | Bioactivity; Biocompatibility; Cell culture; Graphite; Mechanical stability; Nanocomposites; Nanostructures; Reinforcement; Stem cells; Tissue; Tissue engineering; Electrical contacts; Electro-activity; Inflammatory response; Intercellular signaling; Mesenchymal stem cell; Nanocomposite hydrogels; Nerve; Neural tissue engineering; Hydrogels; alginic acid; biomaterial; citric acid; graphite; nanocomposite; nanofiber; animal; cell differentiation; chemistry; cytology; drug effect; electric conductivity; guinea pig; hydrogel; mechanics; nerve regeneration; nervous tissue; PC12 cell line; rat; tissue engineering; Alginates; Animals; Biocompatible Materials; Cell Differentiation; Citric Acid; Electric Conductivity; Graphite; Guinea Pigs; Hydrogels; Mechanical Phenomena; Nanocomposites; Nanofibers; Nerve Regeneration; Nerve Tissue; PC12 Cells; Rats; Tissue Engineering | 公開日期: | 2019 | 卷: | 224 | 來源出版物: | Carbohydrate Polymers | 摘要: | To address the need to biodegradable, electroactive conduits accelerating nerve regeneration, here we develop a nanocomposite hydrogel made of alginate reinforced by citric acid functionalized graphite nanofilaments. The green, simple functionalization enhances the nanofillers distribution and their biocompatibility, as verified using mesenchymal stem cells in vitro. The uniformly distributed nanofilaments raise mechanical stability of the nanocomposite hydrogel versus the neat one up to three times. Also, the nanofilaments enable electrical contact and intercellular signaling thereby stimulating their biological activity. In vitro studies proved the biocompatibility of the nanocomposite hydrogel whereon PC12 cells proliferate and spread evidently. In vivo tests also supported applicability of the nanocomposite hydrogel for implantation within body, and the samples showed no adverse reaction and no inflammatory responses after 14 days. Conclusively, the results certify that the developed electroactive nanocomposite hydrogel is able to stimulate nerve generation and could be confidently used as a nerve conduit material. ? 2019 Elsevier Ltd |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070225636&doi=10.1016%2fj.carbpol.2019.115112&partnerID=40&md5=5430788b15da238a6d999fdbb367fe93 https://scholars.lib.ntu.edu.tw/handle/123456789/577165 |
ISSN: | 1448617 | DOI: | 10.1016/j.carbpol.2019.115112 |
顯示於: | 醫學工程學研究所 |
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