https://scholars.lib.ntu.edu.tw/handle/123456789/578854
標題: | Application of 16S rRNA metagenomics to analyze bacterial communities at a respiratory care centre in Taiwan | 作者: | Tang C.Y. Yiu S.-M. HAN-YUEH KUO Tan T.-S. Liao K.-H. Liu C.-C. Hon W.-K. Liou M.-L. |
關鍵字: | 16S rRNA metagenomics; Acinetobacter; Healthcare-associated infection; Respiratory care center | 公開日期: | 2015 | 出版社: | Springer Verlag | 卷: | 99 | 期: | 6 | 起(迄)頁: | 2871-2881 | 來源出版物: | Applied Microbiology and Biotechnology | 摘要: | In this study, we applied a 16S ribosomal RNA (rRNA) metagenomics approach to survey inanimate hospital environments (IHEs) in a respiratory care center (RCC). A total of 16 samples, including 9 from medical devices and 7 from workstations, were analyzed. Besides, clinical isolates were retrospectively analyzed during the sampling period in the RCC. A high amount of microbial diversity was detected, with an average of 1,836 phylotypes per sample. In addition to Acinetobacter, more than 60?% of the bacterial communities present among the top 25 abundant genera were dominated by skin-associated bacteria. Differences in bacterial profiles were restricted to individual samples. Furthermore, compliance with hand hygiene guidelines may be unsatisfactory among hospital staff according to a principal coordinate analysis that indicated clustering of bacterial communities between devices and workstations for most of the sampling sites. Compared to the high incidence of clinical isolates in the RCC, only Staphylococcus and Acinetobacter were highly abundant in the IHEs. Despite Acinetobacter was the most abundant genus present in IHEs of the RCC, potential pathogens, e.g., Acinetobacter baumannii, might remain susceptible to carbapenem. This study is the first in Taiwan to demonstrate a high diversity of human-associated bacteria in the RCC via 16S rRNA metagenomics, which allows for new assessment of potential health risks in RCCs, aids in the evaluation of existing sanitation protocols, and furthers our understanding of the development of healthcare-associated infections. ? 2014, Springer-Verlag Berlin Heidelberg. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923848665&doi=10.1007%2fs00253-014-6176-7&partnerID=40&md5=ec57e06f3b76e7d2414cef7f22547f0d https://scholars.lib.ntu.edu.tw/handle/123456789/578854 |
ISSN: | 0175-7598 | DOI: | 10.1007/s00253-014-6176-7 | SDG/關鍵字: | Health care; Health risks; Hospitals; Risk assessment; RNA; Acinetobacters; Associated bacteria; Hospital environment; Metagenomics; Microbial diversity; Potential health risks; Principal coordinate analysis; Respiratory care center; Bacteria; carbapenem; RNA 16S; bacterial DNA; carbapenem derivative; RNA 16S; bacterium; genomics; health care; health risk; hospital sector; microbial community; public health; respiratory disease; RNA; sanitation; Acinetobacter baumannii; Article; bacterium isolate; Chryseobacterium; coagulase negative Staphylococcus; hand washing; health hazard; hospital personnel; Klebsiella pneumoniae; medical device; metagenomics; microbial community; microbial diversity; nonhuman; practice guideline; principal coordinate analysis; Pseudomonas aeruginosa; respiratory care; retrospective study; Staphylococcus; Taiwan; allele; bacterium; biomass; classification; DNA sequence; drug effects; Enterococcus; fomite; genetics; human; medical device contamination; metagenomics; microbial sensitivity test; microbiology; multidrug resistance; procedures; Taiwan; Acinetobacter; Acinetobacter baumannii; Bacteria (microorganisms); Staphylococcus; Acinetobacter baumannii; Alleles; Bacteria; Biomass; Carbapenems; Chryseobacterium; DNA, Bacterial; Drug Resistance, Multiple, Bacterial; Enterococcus; Equipment Contamination; Fomites; Humans; Klebsiella pneumoniae; Metagenomics; Microbial Sensitivity Tests; Pseudomonas aeruginosa; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Staphylococcus; Taiwan |
顯示於: | 醫學系 |
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