https://scholars.lib.ntu.edu.tw/handle/123456789/580234
標題: | Dissimilar immunohistochemical expression of ERK and AKT between paired biopsy and hepatectomy tissues of hepatocellular carcinoma | 作者: | YU-YUN SHAO Chen C.-L. MING-CHIH HO Huang C.-C. Tu H.-C. CHIH-HUNG HSU ANN-LII CHENG |
公開日期: | 2012 | 卷: | 32 | 期: | 11 | 起(迄)頁: | 4865-4870 | 來源出版物: | Anticancer Research | 摘要: | Background/Aim: Biomarker studies using immunohistochemical (IHC) staining have not been successful for advanced hepatocellular carcinoma (HCC). We aimed to examine whether the tissue procurement process influences the protein expression levels detected by IHC. Materials and Methods: Forty-two tissue pairs of HCC that had been both preoperatively biopsied and then surgically resected were included in the study. IHC staining was used to determine expression of target molecules, all of which were graded according to the percentage of positively stained tumor cells. The expression of beta-catenin was analyzed according to the localization of positive staining. Results: Biopsied and surgically resected tissues exhibited dissimilar phosphorylated extracellular signal regulated kinase (p-ERK) and phosphorylated AKT expression levels (kappa=0.025 and 0.153, respectively). On the contrary, p53 exhibited similar expression levels, and beta-catenin exhibited similar staining localization patterns in biopsied and surgically resected tissues (kappa=0.729 and 0.654, respectively). Conclusion: Biopsied HCC tissues and their corresponding resected HCC tissues have inconsistent IHC-detected ERK and AKT expressions. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872673927&partnerID=40&md5=f675ca554d70cd12787386a8f4615b41 https://scholars.lib.ntu.edu.tw/handle/123456789/580234 |
SDG/關鍵字: | beta catenin; mitogen activated protein kinase; protein kinase B; protein p53; Wnt protein; article; cancer cell; cancer surgery; clinical article; controlled study; enzyme localization; enzyme phosphorylation; human; human tissue; immunohistochemistry; liver biopsy; liver cell carcinoma; liver resection; preoperative period; priority journal; protein blood level; protein expression; Wnt signaling pathway; Biopsy; Carcinoma, Hepatocellular; Extracellular Signal-Regulated MAP Kinases; Hepatectomy; Humans; Immunohistochemistry; Liver Neoplasms; Proto-Oncogene Proteins c-akt; Tumor Markers, Biological |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。