https://scholars.lib.ntu.edu.tw/handle/123456789/580467
標題: | Transactivation of the human MDR1 gene by hepatitis B virus X gene product | 作者: | SHIN-LIAN DOONG Lin M.-H. Tsai M.-M. Li T.-R. Chuang S.-E. ANN-LII CHENG |
關鍵字: | Hepatitis B virus; Multidrug resistance (MDR); PX | 公開日期: | 1998 | 出版社: | Blackwell Munksgaard | 卷: | 29 | 期: | 6 | 起(迄)頁: | 872-878 | 來源出版物: | Journal of Hepatology | 摘要: | Background/Aims: Persistent hepatitis B virus (HBV) infection may cause hepatocellular carcinoma. Patients with hepatocellular carcinoma are characterized by nonresponsiveness to chemotherapeutic agents. While many studies have been devoted to understanding the hepatocarcinogenesis mechanism of HBV, the possible relationship between HBV and the drug sensitivity phenotype of cancer cells has rarely been addressed. The hepatitis B virus X gene encodes a transcription transactivator which has been suggested to be a potential factor in viral hepatocarcinogenesis. The role of HBV pX in mediating the drug resistance phenotype of hepatoma cell lines was examined in this study. Methods: Standard transfection and chloramphenicol acetyltransferase assay were utilized to examine the effect of HBV pX transactivator on a reporter gene under the control of the human multidrug resistance (MDR) 1 upstream regulatory elements. Selected Hep G2 clones with or without HBV pX expression were tested for their sensitivity towards various anti-cancer agents by utilization of MTT assay. Results: The expression of HBV pX in both Hep G2 (p53+) and Hep 3B (p53-) cells resulted in transactivation of the reporter gene under control of the human MDR1 upstream regulatory elements. Northern blot analysis indicated that expression of the endogenous MDR1 gene was also elevated in Hep G2 clones with HBV pX expression. Decreased drug sensitivity towards adriamycin, vinblastine, and VP-16 was observed in Hep G2 clones with HBV pX expression. Conclusions: HBV pX can transactivate the MDR1 gene. Drug sensitivity was altered in Hep G2 cells with HBV pX expression. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032403863&doi=10.1016%2fS0168-8278%2898%2980113-X&partnerID=40&md5=70c9e6680e619a226cc4b8d68c7ee839 https://scholars.lib.ntu.edu.tw/handle/123456789/580467 |
ISSN: | 0168-8278 | DOI: | 10.1016/S0168-8278(98)80113-X | SDG/關鍵字: | doxorubicin; etoposide; fluorouracil; gene product; transactivator protein; vinblastine; virus protein; article; drug sensitivity; gene expression; hepatitis B; Hepatitis B virus; human; human cell; liver carcinogenesis; liver cell carcinoma; multidrug resistance; priority journal; transactivation; virus carcinogenesis |
顯示於: | 醫學系 |
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