Establishment of an immunocompetent metastasis rat model with hepatocyte cancer stem cells
Journal
Cancers
Journal Volume
12
Journal Issue
12
Pages
1-14
Date Issued
2020
Author(s)
Abstract
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer mortality. Cancer stem cells (CSCs) are responsible for the maintenance, metastasis, and relapse of various tumors. The effects of CSCs on the tumorigenesis of HCC are still not fully understood, however. We have recently established two new rat HCC cell lines HTC and TW-1, which we isolated from diethylnitrosamine-induced rat liver cancer. Results showed that TW-1 expressed the genetic markers of CSCs, including CD133, GSTP1, CD44, CD90, and EpCAM. Moreover, TW-1 showed higher tolerance to sorafenib than HTC did. In addition, tumorigenesis and metastasis were observed in nude mice and wild-type rats with TW-1 xenografts. Finally, we combined highly expressed genes in TW-1/HTC with well-known biomarkers from recent HCC studies to predict HCC-related biomarkers and able to identify HCC with AUCs > 0.9 after machine learning. These results indicated that TW-1 was a novel rat CSC line, and the mice or rat models we established with TW-1 has great potential on HCC studies in the future. ? 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
CD133 antigen; epithelial cell adhesion molecule; glutathione transferase P1; Hermes antigen; retrorsine; sorafenib; Thy 1 membrane glycoprotein; animal cell; animal experiment; animal model; animal tissue; Article; cancer stem cell; cell differentiation; cell self-renewal; concentration response; controlled study; disease marker; drug screening; gene expression; HTC cell line (hepatocellular carcinoma); immunocompromised patient; immunofluorescence; in vitro study; in vivo study; incubation time; liver cell; liver cell carcinoma; logistic regression analysis; machine learning; male; metastasis potential; microarray analysis; mitochondrial respiration; nonhuman; random forest; rat; staining; support vector machine; TW 1 cell line; Western blotting
SDGs
Other Subjects
CD133 antigen; epithelial cell adhesion molecule; glutathione transferase P1; Hermes antigen; retrorsine; sorafenib; Thy 1 membrane glycoprotein; animal cell; animal experiment; animal model; animal tissue; Article; cancer stem cell; cell differentiation; cell self-renewal; concentration response; controlled study; disease marker; drug screening; gene expression; HTC cell line (hepatocellular carcinoma); immunocompromised patient; immunofluorescence; in vitro study; in vivo study; incubation time; liver cell; liver cell carcinoma; logistic regression analysis; machine learning; male; metastasis potential; microarray analysis; mitochondrial respiration; nonhuman; random forest; rat; staining; support vector machine; TW 1 cell line; Western blotting
Type
journal article