https://scholars.lib.ntu.edu.tw/handle/123456789/582412
標題: | Functionalizing Collagen with Vessel-Penetrating Two-Photon Phosphorescence Probes: A New In Vivo Strategy to Map Oxygen Concentration in Tumor Microenvironment and Tissue Ischemia | 作者: | Wu, Cheng-Ham Kisel, Kristina S Thangavel, Muthu Kumar Chen, Yi-Ting Chang, Kai-Hsin Tsai, Ming-Rung CHIA-YU CHU Shen, Yu-Fang Wu, Pei-Chun Zhang, Zhiming Liu, Tzu-Ming Jänis, Janne Grachova, Elena V Shakirova, Julia R Tunik, Sergey P Koshevoy, Igor O PI-TAI CHOU |
關鍵字: | ReI diimine carbonyl complexes; phosphorescence lifetime imaging microscopy; phosphorescent oxygen sensors; tissue ischemia; tumor hypoxia; two-photon phosphorescence | 公開日期: | 19-八月-2021 | 來源出版物: | Advanced science | 摘要: | The encapsulation and/or surface modification can stabilize and protect the phosphorescence bio-probes but impede their intravenous delivery across biological barriers. Here, a new class of biocompatible rhenium (ReI ) diimine carbonyl complexes is developed, which can efficaciously permeate normal vessel walls and then functionalize the extravascular collagen matrixes as in situ oxygen sensor. Without protective agents, ReI -diimine complex already exhibits excellent emission yield (34%, λem = 583 nm) and large two-photon absorption cross-sections (σ2 = 300 GM @ 800 nm) in water (pH 7.4). After extravasation, remarkably, the collagen-bound probes further enhanced their excitation efficiency by increasing the deoxygenated lifetime from 4.0 to 7.5 µs, paving a way to visualize tumor hypoxia and tissue ischemia in vivo. The post-extravasation functionalization of extracellular matrixes demonstrates a new methodology for biomaterial-empowered phosphorescence sensing and imaging. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/582412 | ISSN: | 2198-3844 | DOI: | 10.1002/advs.202102788 |
顯示於: | 醫學系 |
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