https://scholars.lib.ntu.edu.tw/handle/123456789/582865
標題: | Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in Arabidopsis | 作者: | Hu, Shu Hsuan Lin, Shu Fan Huang, Ya Chen Huang, Chien Hsun Kuo, Wen Yu TSUNG-LUO JINN |
關鍵字: | metalloenzyme | mitochondrial carrier family | Mn transporter | MnSOD | reactive oxygen species | superoxide dismutase | 公開日期: | 6-八月-2021 | 出版社: | Frontiers Media S.A. | 卷: | 12 | 期: | Article number 690064 | 來源出版物: | Frontiers in Plant Science | 摘要: | The manganese (Mn) tracking factor for mitochondrial Mn superoxide dismutase (MnSOD) has been annotated as yMTM1 in yeast, which belongs to the mitochondrial carrier family. We confirmed that Arabidopsis AtMTM1 and AtMTM2 are functional homologs of yMTM1 as they can revive yeast MnSOD activity in yMTM1-mutant cells. Transient expression of AtMnSOD-3xFLAG in the AtMTM1 and AtMTM2-double mutant protoplasts confirmed that AtMTM1 and AtMTM2 are required for AtMnSOD activation. Our study revealed that AtMnSOD interacts with AtMTM1 and AtMTM2 in the mitochondria. The expression levels of AtMTM1, AtMTM2, and AtMnSOD respond positively to methyl viologen (MV) and metal stress. AtMTM1 and AtMTM2 are involved in Mn and Fe homeostasis, root length, and flowering time. Transient expression of chloroplast-destined AtMnSOD revealed that an evolutionarily conserved activation mechanism, like the chloroplastic-localized MnSOD in some algae, still exists in Arabidopsis chloroplasts. This study strengthens the proposition that AtMTM1 and AtMTM2 participate in the AtMnSOD activation and ion homeostasis. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/582865 | ISSN: | 1664462X | DOI: | 10.3389/fpls.2021.690064 |
顯示於: | 植物科學研究所 |
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