https://scholars.lib.ntu.edu.tw/handle/123456789/582935
標題: | ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer | 作者: | Cheung, C.H.Y. CHIA-LANG HSU Lin, T.-Y. Chen, W.-T. Wang, Y.-C. Huang, H.-C. Juan, H.-F. |
公開日期: | 2020 | 出版社: | BioMed Central | 卷: | 27 | 期: | 1 | 起(迄)頁: | 75 | 來源出版物: | Journal of Biomedical Science | 摘要: | Background: ZNF322A is an oncogenic transcription factor that belongs to the Cys2His2-type zinc-finger protein family. Accumulating evidence suggests that ZNF322A may contribute to the tumorigenesis of lung cancer, however, the ZNF322A-mediated downstream signaling pathways remain unknown. Methods: To uncover ZNF322A-mediated functional network, we applied phosphopeptide enrichment and isobaric labeling strategies with mass spectrometry-based proteomics using A549 lung cancer cells, and analyzed the differentially expressed proteins of phosphoproteomic and proteomic profiles to determine ZNF322A-modulated pathways. Results: ZNF322A highlighted a previously unidentified insulin signaling, heat stress, and signal attenuation at the post-translational level. Consistently, protein-phosphoprotein-kinase interaction network analysis revealed phosphorylation of IRS1 and HSP27 were altered upon ZNF322A-silenced lung cancer cells. Thus, we further investigated the molecular regulation of ZNF322A, and found the inhibitory transcriptional regulation of ZNF322A on PIM3, which was able to phosphorylate IRS1 at serine1101 in order to manipulate glucose uptake via the PI3K/AKT/mTOR signaling pathway. Moreover, ZNF322A also affects the unfolded protein response by phosphorylation of HSP27S82 and eIF2aS51, and triggers autophagosome formation in lung cancer cells. Conclusions: These findings not only give new information about the molecular regulation of the cellular proteins through ZNF322A at the post-translational level, but also provides a resource for the study of lung cancer therapy. ? 2020 The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087023680&doi=10.1186%2fs12929-020-00668-5&partnerID=40&md5=648311263b16a0fec3f5000a4f8c352f https://scholars.lib.ntu.edu.tw/handle/123456789/582935 |
ISSN: | 1021-7770 | DOI: | 10.1186/s12929-020-00668-5 | SDG/關鍵字: | heat shock protein 27; initiation factor 2alpha; insulin receptor substrate 1; protein kinase B; serine; transcription factor; transcription factor ZNF322A; unclassified drug; heat shock protein; insulin receptor substrate; IRS1 protein, human; oncoprotein; protein kinase B; transcription factor; ZNF322a protein, human; A-549 cell line; Akt signaling; Article; autophagosome; controlled study; glucose transport; heat stress; human; human cell; insulin signaling; lung cancer; mass spectrometry; phosphoproteomics; priority journal; protein expression; protein phosphorylation; protein protein interaction; transcription regulation; unfolded protein response; autophagosome; genetics; lung tumor; metabolism; phosphorylation; unfolded protein response; A549 Cells; Autophagosomes; Heat-Shock Proteins; Humans; Insulin Receptor Substrate Proteins; Lung Neoplasms; Oncogene Proteins; Phosphorylation; Proto-Oncogene Proteins c-akt; Transcription Factors; Unfolded Protein Response |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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