Diet supplementation of Pediococcus pentosaceus in cobia (Rachycentron canadum) enhances growth rate, respiratory burst andresistance against photobacteriosis
Journal
Fish and Shellfish Immunology
Journal Volume
35
Journal Issue
4
Pages
1122-1128
Date Issued
2013
Author(s)
Abstract
Cobia ( Rachycentron canadum) is an economically important fish species for aquaculture in tropical and sub-tropical areas. Cobia aquaculture industry has severely damaged due to photobacteriosis caused by Photobacterium damselae subsp. piscicida ( Pdp), especially in Taiwan. Antibiotics and vaccines have been applied to control Pdp infection, but the efficacy has been inconsistent. One species of lactic acid bacteria, Pediococcus pentosaceus strain 4012 (LAB 4012), was isolated from the intestine of adult cobia, and its culture supernatant can effectively inhibit Pdp growth invitro. The acidic pH derived from metabolic acids in LAB culture supernatant was demonstrated to be an important factor for the suppression. After a 2-week feeding of LAB 4012, the growth rate of the fed cobia was 12% higher than that of the non-fed group, and the relative percentage of survival (RPS) of the fed cobia was found to be 74.4 in Pdp immersion challenge. In addition, the respiratory burst (RB) of peripheral blood leukocytes (PBL) in the LAB 4012-fed group was significantly higher than that of the non-fed group. Although feeding LAB 4012 did not improve specific antibody response in cobia after immunization with Pdp vaccine, it still significantly raised the survival rate by 22% over that of the non-fed group after Pdp immersion challenge. Judging by the quick induction of high protection against Pdp infection and promotion of growth in larvae, LAB 4012 was considered to be a viable probiotic for cobia aquaculture. ? 2013 Elsevier Ltd.
SDGs
Other Subjects
Bacteria (microorganisms); Pediococcus pentosaceus; Photobacterium damselae; Photobacterium damselae subsp. piscicida; Rachycentron canadum; bacterial vaccine; probiotic agent; animal; animal disease; animal food; aquaculture; article; Cobia; controlled clinical trial; controlled study; diet; fish disease; Gram negative infection; growth, development and aging; immunology; lactic acid bacterium; larva; microbiology; Pediococcus; Perciformes; Photobacteriosis; Photobacterium; Photobacterium damselae subsp. piscicida; physiology; randomized controlled trial; respiratory burst; Cobia; Lactic acid bacteria; Photobacteriosis; Photobacterium damselae subsp. piscicida; Probiotics; Animal Feed; Animals; Aquaculture; Bacterial Vaccines; Diet; Fish Diseases; Gram-Negative Bacterial Infections; Larva; Pediococcus; Perciformes; Photobacterium; Probiotics; Respiratory Burst
Publisher
Academic Press
Type
journal article