https://scholars.lib.ntu.edu.tw/handle/123456789/585051
標題: | 5-aminolevulinic acid induce apoptosis via NF-κB/JNK pathway in human oral cancer Ca9-22 cells | 作者: | HSIN-MING CHEN CHEING-MEEI LIU Yang H. HAN-YI E. CHOU CHUN-PIN CHIANG YEN-PING KUO |
公開日期: | 2011 | 卷: | 40 | 期: | 6 | 起(迄)頁: | 483-489 | 來源出版物: | Journal of Oral Pathology and Medicine | 摘要: | 5-aminolevulinic acid-based photodynamic therapy (5-ALA-PDT) is being used to treat oral pre-cancerous and cancerous lesions with some encouraging clinical outcomes. However, the exact mechanisms behind the photodynamic treatment are still not fully elucidated. Method: Flow cytometry, TdT-mediated dUTP nick end labeling assay and Western blot analysis were used to investigate the effects of 5-ALA-PDT on human oral cancer Ca9-22 cells. Results: We found that 5-ALA-PDT induces apoptosis in Ca9-22 cells. Western blotting showed that 5-ALA-PDT activates both the caspase-8 and caspase-9 pathways, which differed from previous studies conducted in other cell types. Activation of JNK was evident as early as 30min. The caspases activation was inhibited by JNK inhibitor SP600125. Treatment with NF-κB inhibitor Bay 11-7082 (Bay) completely abrogated ALA-PDT-induced JNK activation. In addition, Bay and SP600125 almost completely abolished ALA-PDT-induced apoptosis. Conclusion: These results demonstrate significant involvement of caspase-8 and -9 and their upstream NF-κB-JNK pathways in ALA-PDT-induced apoptosis. Future studies on how NF-κB and JNK activity regulate ALA-PDT response should provide a better strategy for the treatment of oral cancer. ? 2010 John Wiley & Sons A/S. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960006993&doi=10.1111%2fj.1600-0714.2010.00973.x&partnerID=40&md5=c958b3b6d5b91c46281d4d89ba6b9258 https://scholars.lib.ntu.edu.tw/handle/123456789/585051 |
ISSN: | 0904-2512 | DOI: | 10.1111/j.1600-0714.2010.00973.x | SDG/關鍵字: | aminolevulinic acid; caspase 8; caspase 9; DNA nucleotidylexotransferase; immunoglobulin enhancer binding protein; mitogen activated protein kinase; stress activated protein kinase; apoptosis; article; clinical effectiveness; controlled study; enzyme activation; enzyme linked immunosorbent assay; flow cytometry; human; human cell; mouth cancer; nick end labeling; photodynamic therapy; priority journal; protein phosphorylation; Western blotting; Aminolevulinic Acid; Apoptosis; Caspase 8; Caspase 9; Cell Line, Tumor; Fas-Associated Death Domain Protein; Humans; In Situ Nick-End Labeling; JNK Mitogen-Activated Protein Kinases; MAP Kinase Signaling System; Mouth Neoplasms; NF-kappa B; Photochemotherapy; RNA Interference |
顯示於: | 牙醫學系 |
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