https://scholars.lib.ntu.edu.tw/handle/123456789/585076
標題: | Time-resolved autofluorescence spectroscopy for classifying normal and premalignant oral tissues | 作者: | HSIN-MING CHEN CHUN-PIN CHIANG You C. Hsiao T.-C. Wang C.-Y. |
公開日期: | 2005 | 卷: | 37 | 期: | 1 | 起(迄)頁: | 37-45 | 來源出版物: | Lasers in Surgery and Medicine | 摘要: | Background and Objectives: Time-resolved autofluorescence spectroscopy has been used for effectively distinguishing normal tissues from precancers and cancers in various organs. The aim of this study was to find out the possibility of using time-resolved autofluorescence spectroscopy to differentiate normal oral mucosa (NOM) from oral premalignant lesions including verrucous hyperplasia (VH), epithelial hyperplasia (EH), and epithelial dysplasia (ED). Study Design/Materials and Methods: Time-resolved autofluorescence spectra at 633 nm under 410-nm excitation were recorded for 15 VH, 9 EH, 14 ED, and 38 NOM samples. The two-component lifetimes of the obtained curves were calculated, and a Fisher's discriminant analysis (FDA) was employed for distinguishing these tissue samples. Results: After two-component lifetimes for all samples being calculated, a two-dimensional scatter plot was developed, in which 76 oral tissue samples were separated into three groups by FDA. With a leave-one-out method, the FDA algorithm gave an accuracy rate of 93% for ED, of 75% for VH and EH, and of 100% for NOM samples. In addition, all oral premalignant lesions (including VH, EH, and ED) could be distinguished from NOM samples by this FDA algorithm. Conclusions: We conclude that time-resolved autofluorescence spectroscopy at 633 nm under 410-nm excitation, based on two-component lifetime calculation and FDA, is a very sensitive technique for in vivo diagnosis of oral pre-malignant lesions. ? 2005 Wiley-Liss, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-23344445932&doi=10.1002%2flsm.20192&partnerID=40&md5=d4cb72d86e482e29b835bac5519e1375 https://scholars.lib.ntu.edu.tw/handle/123456789/585076 |
ISSN: | 0196-8092 | DOI: | 10.1002/lsm.20192 | SDG/關鍵字: | fluorescence lifetime; unclassified drug; accuracy; adult; aged; algorithm; article; autofluorescence; classification; clinical article; discriminant analysis; epithelium dysplasia; epithelium hyperplasia; female; Fisher discriminant analysis; fluorescence; fluorescence spectroscopy; histopathology; human; human tissue; hyperplasia; male; mouth; precancer; priority journal; radiobiology; time resolved autofluorescence spectroscopy; verrucous hyperplasia; Adult; Aged; Female; Humans; Male; Middle Aged; Mouth Mucosa; Mouth Neoplasms; Precancerous Conditions; Spectrometry, Fluorescence |
顯示於: | 牙醫學系 |
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