https://scholars.lib.ntu.edu.tw/handle/123456789/586009
標題: | CRMP/UNC-33 organizes microtubule bundles for KIF5-mediated mitochondrial distribution to axon | 作者: | Chen, Ying-Chun Huang, Hao-Ru Hsu, Chia-Hao CHAN-YEN OU |
公開日期: | 2021 | 卷: | 17 | 期: | 2 | 來源出版物: | PLoS genetics | 摘要: | Neurons are highly specialized cells with polarized cellular processes and subcellular domains. As vital organelles for neuronal functions, mitochondria are distributed by microtubule-based transport systems. Although the essential components of mitochondrial transport including motors and cargo adaptors are identified, it is less clear how mitochondrial distribution among somato-dendritic and axonal compartment is regulated. Here, we systematically study mitochondrial motors, including four kinesins, KIF5, KIF17, KIF1, KLP-6, and dynein, and transport regulators in C. elegans PVD neurons. Among all these motors, we found that mitochondrial export from soma to neurites is mainly mediated by KIF5/UNC-116. Interestingly, UNC-116 is especially important for axonal mitochondria, while dynein removes mitochondria from all plus-end dendrites and the axon. We surprisingly found one mitochondrial transport regulator for minus-end dendritic compartment, TRAK-1, and two mitochondrial transport regulators for axonal compartment, CRMP/UNC-33 and JIP3/UNC-16. While JIP3/UNC-16 suppresses axonal mitochondria, CRMP/UNC-33 is critical for axonal mitochondria; nearly no axonal mitochondria present in unc-33 mutants. We showed that UNC-33 is essential for organizing the population of UNC-116-associated microtubule bundles, which are tracks for mitochondrial trafficking. Disarrangement of these tracks impedes mitochondrial transport to the axon. In summary, we identified a compartment-specific transport regulation of mitochondria by UNC-33 through organizing microtubule tracks for different kinesin motors other than microtubule polarity. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/586009 | ISSN: | 15537390 | DOI: | 10.1371/journal.pgen.1009360 |
顯示於: | 生物化學暨分子生物學科研究所 |
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