https://scholars.lib.ntu.edu.tw/handle/123456789/586638
Title: | Investigation of the Number of Tests Required for Assaying Plasma Biomarkers Associated with Alzheimer’s Disease Using Immunomagnetic Reduction | Authors: | Liu H.-C. Chen H.-H. Ho C.-S. Chang J.-F. Lin C.-C. MING-JANG CHIU TA-FU CHEN Hu C.-J. Yan S.-H. Sun Y. Yang S.-Y. |
Issue Date: | 2021 | Source: | Neurology and Therapy | Abstract: | Introduction: Concentrations of plasma biomarkers associated with Alzheimer’s disease have been reported to be as low as several tens of picograms/milliliter (pg/ml). However, in assays measuring these biomarkers, it is likely that repeated measurements are necessary to obtain reliable values. Methods: We performed assays as a single test or as duplicate, quadruplicate, fivefold and tenfold repeated tests, on samples spiked with different concentrations of amyloid β 1–40 (Aβ1–40; 1–1000?pg/ml), Aβ1–42 (1–30,000?pg/ml) and total Tau protein (T-Tau; 0.1–1000?pg/ml), with the aim to to calculate the coefficients of variation (CVs). Results: The results demonstrated common changes in the CVs with changes in the number of tests for a given sample: the CVs decreased with increases in the number of tests from one to ten. All CV values were distributed within the range of 0.35 to 15.5%; as such, the CV values were all lower than the acceptable value of 20%. Conclusion: Based on this study, a single assay of Aβ1–40, Aβ1–42 and T-Tau, respectively, provides reliable results in terms of the measurement of that plasma biomarker. ? 2021, The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114775251&doi=10.1007%2fs40120-021-00280-1&partnerID=40&md5=4ca2d7d562e592cfecbee20f55fb5215 https://scholars.lib.ntu.edu.tw/handle/123456789/586638 |
ISSN: | 21938253 | DOI: | 10.1007/s40120-021-00280-1 | SDG/Keyword: | amyloid beta protein; amyloid beta protein[1-40]; amyloid beta protein[1-42]; biological marker; tau protein; aged; Alzheimer disease; Article; blood sampling; brain size; controlled study; female; Geriatric Depression Scale; hippocampus; human; immunomagnetic separation; major clinical study; male; mathematical model; measurement repeatability; mild cognitive impairment; Mini Mental State Examination; neuropsychological test; plasma; protein blood level; volume [SDGs]SDG3 |
Appears in Collections: | 醫學院附設醫院 (臺大醫院) |
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