https://scholars.lib.ntu.edu.tw/handle/123456789/586666
標題: | A real-time mirror-LAPS mini system for dynamic chemical imaging and cell acidification monitoring | 作者: | Yang, Chia Ming Yen, Tzung‐Hai H. Liu, Hui Ling Lin, Yu Jing Lin, Po Yen Tsui, Leung Sze Chen, Chun Hui YU-PING CHEN Hsu, Yu Chieh Lo, Chih Hong HSIN-CHIH LAI Wu, Tsung Ru Chin, Wei Chun Pijanowska, Dorota G. Hwang, Tsann Long Lai, Chao Sung |
關鍵字: | 2D imaging | Acidification | Analog micromirror | Cell | FPGA | LAPS | 公開日期: | 15-八月-2021 | 卷: | 341 | 來源出版物: | Sensors and Actuators, B: Chemical | 摘要: | A fully integrated system and user interface, named Mirror-LAPS, was established for chemical imaging of diffusion-based reactions and bioimaging, e.g., cellular metabolism imaging in situ, with advantages of portability, real-time monitoring and flexible control. By means of a field programmable gate array (FPGA), a minimized drive circuit and an illumination path integrated into the hardware, the volume and cost of this Mirror-LAPS system are only 11 % and 75 %, respectively, those of the conventional LAPS system. 2D images can be easily constructed based on the combination of the measured photocurrent with automatic digitization and recorded coordinates for each pixel in the frame. The time to image one pixel under the optimized parameters with a periods per pixel (PPP) of 64 is approximately 6.6 ms. The location, area, and resolution of pixels can be easily, quickly and flexibly adjusted in the user interface to obtain 2D images and a real-time video. The cellular metabolism of HK2 cellsfor different cell numbers and glucose concentrations is virtually demonstrated by this Mirror-LAPS system by the color changes in 2D images for the first time, which could provide a reliable platform in a cell culturing space for cell-based research. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/586666 | ISSN: | 09254005 | DOI: | 10.1016/j.snb.2021.130003 |
顯示於: | 醫學檢驗暨生物技術學系 |
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