Wavelength coded volume holographic gratings based dual wavelength fluorescence imaging system
Journal
Proceedings of SPIE - The International Society for Optical Engineering
Journal Volume
11709
Pages
117090U
Date Issued
2021
Author(s)
Abstract
We present a wavelength coded volume holographic gratings (WC-VHGs) based dual wavelength fluorescence imaging system to simultaneously obtain two color fluorescence images of biological samples in one shot. The system utilizes wavelength coded recording technique based on Bragg degeneracy to generate the PQ-PMMA volume holographic gratings, which has high selectivity in both wavelength and angle. In order to improve the optical sectioning ability of the proposed system, we use HiLo image processing to suppress the out of focus signal. Experimental results demonstrate the imaging ability of the proposed system to observe multi-wavelength fluorescence images and HiLo images.
Publisher
SPIE
Type
conference paper