https://scholars.lib.ntu.edu.tw/handle/123456789/589201
標題: | The use of chitosan to enhance photodynamic inactivation against Candida albicans and its drug-resistant clinical isolates | 作者: | Chien H.-F. Chen C.-P. YEE-CHUN CHEN Chang P.-H. Tsai T. Chen C.-T. |
公開日期: | 2013 | 卷: | 14 | 期: | 4 | 起(迄)頁: | 7445-7456 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | Drug-resistant Candida infection is a major health concern among immunocompromised patients. Antimicrobial photodynamic inactivation (PDI) was introduced as an alternative treatment for local infections. Although Candida (C.) has demonstrated susceptibility to PDI, high doses of photosensitizer (PS) and light energy are required, which may be harmful to eukaryotic human cells. This study explores the capacity of chitosan, a polycationic biopolymer, to increase the efficacy of PDI against C. albicans, as well as fluconazole-resistant clinical isolates in planktonic or biofilm states. Chitosan was shown to effectively augment the effect of PDI mediated by toluidine blue O (TBO) against C. albicans that were incubated with chitosan for 30 min following PDI. Chitosan at concentrations as low as 0.25% eradicated C. albicans; however, without PDI treatment, chitosan alone did not demonstrate significant antimicrobial activity within the 30 min of incubation. These results suggest that chitosan only augmented the fungicidal effect after the cells had been damaged by PDI. Increasing the dosage of chitosan or prolonging the incubation time allowed a reduction in the PDI condition required to completely eradicate C. albicans. These results clearly indicate that combining chitosan with PDI is a promising antimicrobial approach to treat infectious diseases. ? 2013 by the authors; licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875979378&doi=10.3390%2fijms14047445&partnerID=40&md5=81ca2895d23ff2969f4fe1e78c2ac9a4 https://scholars.lib.ntu.edu.tw/handle/123456789/589201 |
ISSN: | 1661-6596 | DOI: | 10.3390/ijms14047445 | SDG/關鍵字: | chitosan; fluconazole; photosensitizing agent; tolonium chloride; chitosan; coloring agent; photosensitizing agent; tolonium chloride; antifungal resistance; article; binding assay; biofilm; Candida albicans; cell damage; cell survival; cell viability; concentration response; controlled study; drug effect; drug potentiation; fungal cell; fungal strain; fungal structures; fungicidal activity; fungus isolation; incubation time; light exposure; monotherapy; nonhuman; photodynamic inactivation; photodynamic therapy; plankton; biofilm; Candida albicans; candidiasis; drug effects; human; isolation and purification; photochemotherapy; physiology; Candida; Candida albicans; Eukaryota; Biofilms; Candida albicans; Candidiasis; Chitosan; Coloring Agents; Drug Resistance, Fungal; Humans; Photochemotherapy; Photosensitizing Agents; Tolonium Chloride |
顯示於: | 醫學系 |
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