https://scholars.lib.ntu.edu.tw/handle/123456789/589890
標題: | Identification of a gut microbiota member that ameliorates DSS-induced colitis in intestinal barrier enhanced Dusp6-deficient mice | 作者: | Chang, Cherng Shyang Liao, Yi Chu Huang, Chih Ting Lin, Chiao Mei Cheung, Chantal Hoi Yin Ruan, Jhen Wei Yu, Wen Hsuan Tsai, Yi Ting Lin, I. Jung Huang, Chien Hsun Liou, Jong Shian Chou, Ya Hsien Chien, Hung Jen Chuang, Hsiao Li HSUEH-FEN JUAN Huang, Hsuan Cheng Chan, Hong Lin Liao, Yu Chieh Tang, Shiue Cheng Su, Yu Wen Tan, Tse Hua Bäumler, Andreas J. Kao, Cheng Yuan |
關鍵字: | barrier integrity | DUSP6 | experimental colitis | gut microbiota | leaky gut;barrier integrity; DUSP6; experimental colitis; gut microbiota; leaky gut | 公開日期: | 23-十一月-2021 | 出版社: | Elsevier B.V. | 卷: | 37 | 期: | 8 | 起(迄)頁: | 110016 | 來源出版物: | Cell Reports | 摘要: | Strengthening the gut epithelial barrier is a potential strategy for management of gut microbiota-associated illnesses. Here, we demonstrate that dual-specificity phosphatase 6 (Dusp6) knockout enhances baseline colon barrier integrity and ameliorates dextran sulfate sodium (DSS)-induced colonic injury. DUSP6 mutation in Caco-2 cells enhances the epithelial feature and increases mitochondrial oxygen consumption, accompanied by altered glucose metabolism and decreased glycolysis. We find that Dusp6-knockout mice are more resistant to DSS-induced dysbiosis, and the cohousing and fecal microbiota transplantation experiments show that the gut/fecal microbiota derived from Dusp6-knockout mice also confers protection against colitis. Further culturomics and mono-colonialization experiments show that one gut microbiota member in the genus Duncaniella confers host protection from DSS-induced injury. We identify Dusp6 deficiency as beneficial for shaping the gut microbiota eubiosis necessary to protect against gut barrier-related diseases. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/589890 | ISSN: | 22111247 | DOI: | 10.1016/j.celrep.2021.110016 | SDG/關鍵字: | dual specificity phosphatase 6; transcriptome; aged; animal cell; Article; bacterium identification; Caco-2 cell line; clinical article; colitis; colon epithelium; controlled study; dextran sulfate sodium-induced colitis; Dusp6 gene; dysbiosis; fecal microbiota transplantation; gene identification; gene knockout; gene mutation; glucose metabolism; glycolysis; histology; human; human cell; intestine flora; male; microvillus; mitochondrion; mouse; nonhuman; oxygen consumption; protein phosphorylation; real time polymerase chain reaction; RNA sequencing; tight junction; Western blotting |
顯示於: | 分子與細胞生物學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。