https://scholars.lib.ntu.edu.tw/handle/123456789/589915
標題: | Ultrasensitive Detection of Alzheimer's Amyloids on a Plasmonic-Gold Platform | 作者: | Han, Hsiao Fen Yen, Hung Chi Wu, Hsiu Chuan Tan, Hsin Yuan Xu, Wei Jiang, Hang Shiang Tsai, Ping Jui Qian, Kun YI-CHUN WU Chen, Chia Chun |
關鍵字: | Alzheimer's disease | beta-amyloid | blood biomarkers | plasmonic-gold chip | surface plasmon-enhanced fluorescence | 公開日期: | 1-一月-2021 | 出版社: | American Chemical Society | 卷: | 13 | 期: | 48 | 起(迄)頁: | 57036-57042 | 來源出版物: | ACS Applied Materials and Interfaces | 摘要: | More than 55 million people live with dementia worldwide in 2021, and there are nearly 10 million new cases every year. Alzheimer's disease (AD) is the most common cause of dementia. Despite urgent need, early detection of AD and long-term monitoring of AD progression have been challenging. This is due to the limited availability of brain imaging facilities and the highly invasive procedure with the cerebrospinal fluid assay to assess the level of AD biomarkers, such as beta-amyloid (Aβ). Reliable measurements of AD biomarkers in blood samples are still difficult because of their very low abundance. Here, we develop a rapid, specific, and ultrasensitive immunoassay using plasmonic-gold nanoisland (pGOLD) chips with near-infrared fluorescence-enhanced detection for Aβ1-40 and Aβ1-42. We show step-by-step processes and results during the platform establishment, including antibody specificity and sensitivity tests, antibody pair examination, condition optimization, and procedure refinement. Finally, we demonstrate the platform performance with detection sensitivity at the subpicogram per milliliter level. This platform, therefore, has a great application potential for early detection of AD using blood samples. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/589915 | ISSN: | 19448244 | DOI: | 10.1021/acsami.1c19157 |
顯示於: | 分子與細胞生物學研究所 |
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