Molecular cloning of a cold-shock domain protein, zfY1, in zebrafish embryo
Journal
Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Journal Volume
1433
Journal Issue
1-2
Pages
2103-2116
Date Issued
1999
Author(s)
Abstract
Cold-shock domain proteins in vertebrates contain a highly conserved domain which is related to the Escherichia coli cold-shock proteins. Here we report the cloning of a cold-shock domain protein from zebrafish embryo. Using the combination of PCR techniques with degenerate primers, 5'RACE and 3'RACE, the full length cDNA of a cold-shock domain protein in the zebrafish embryo was successfully cloned without constructing and screening a library. Determined from the deduced amino acid sequence, this protein is most similar to Xenopus, FRGY1, and this newly cloned zebrafish gene was therefore designated as zfY1.
Publisher
Elsevier
Type
journal article