https://scholars.lib.ntu.edu.tw/handle/123456789/591843
標題: | The influence of a caveolin-1 mutant on the function of P-glycoprotein | 作者: | CHIH-YUAN LEE Lai T.-Y. MENG-KUN TSAI Ou-Yang P. Tsai C.-Y. Wu S.-W. Hsu L.-C. JIN-SHING CHEN |
公開日期: | 2016 | 出版社: | Nature Publishing Group | 卷: | 6 | 來源出版物: | Scientific Reports | 摘要: | The genetic heterogeneity in cancer cells has an increased chance in the acquisition of new mutant such as drug-resistant phenotype in cancer cells. The phenotype of drug resistance in cancer cells could be evaluated by the number or function of drug transporters on cell membranes, which would lead to decreased intracellular anti-cancer drugs concentration. Caveolae are flask-shaped invaginations on cell membrane that function in membrane trafficking, endocytosis, and as a compartment where receptors and signaling proteins are concentrated. Caveolin-1 (CAV1) is the principal structural protein of caveolae and closely correlates with multidrug resistance in cancer cells. In a systematic study of the ubiquitin-modified proteome, lysine 176 of CAV1 was identified as a potential post-translational modification site for ubiquitination. In this article, we identified a mutation at lysine 176 to arginine (K176R) on CAV1 would interfere with the biogenesis of caveolae and broke the interaction of CAV1 with P-glycoprotein. Functional assays further revealed that K176R mutant of CAV1 in cancer cells increased the transport activity of P-glycoprotein and decreased the killing ability of anti-cancer drugs in non-small-cell lung cancer cell lines. ? 2016, Nature Publishing Group. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957597064&doi=10.1038%2fsrep20486&partnerID=40&md5=1fff90674dc9abd7d20624962e013124 https://scholars.lib.ntu.edu.tw/handle/123456789/591843 |
ISSN: | 2045-2322 | DOI: | 10.1038/srep20486 | SDG/關鍵字: | ABCB1 protein, human; CAV1 protein, human; caveolin 1; doxorubicin; lysine; multidrug resistance protein; cell survival; chemistry; drug effects; drug resistance; genetics; human; lung tumor; metabolism; mutation; non small cell lung cancer; protein multimerization; protein processing; protein transport; tumor cell line; Carcinoma, Non-Small-Cell Lung; Caveolin 1; Cell Line, Tumor; Cell Survival; Doxorubicin; Drug Resistance, Neoplasm; Humans; Lung Neoplasms; Lysine; Mutation; P-Glycoproteins; Protein Multimerization; Protein Processing, Post-Translational; Protein Transport |
顯示於: | 醫學系 |
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