|Title:||Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cells||Authors:||Wu, David
Harrison, Devin L
Mutlu, Gökhan M
|Keywords:||RESONANCE ENERGY-TRANSFER; RHO KINASE; THROMBIN; PROLIFERATION; TRANSLOCATION; ACTIVATION; EXPRESSION; MIGRATION; DYNAMICS||Issue Date:||May-2021||Publisher:||NATURE RESEARCH||Journal Volume:||3||Journal Issue:||5||Start page/Pages:||714||Source:||Nature metabolism||Abstract:||
Single-cell motility is spatially heterogeneous and driven by metabolic energy. Directly linking cell motility to cell metabolism is technically challenging but biologically important. Here, we use single-cell metabolic imaging to measure glycolysis in individual endothelial cells with genetically encoded biosensors capable of deciphering metabolic heterogeneity at subcellular resolution. We show that cellular glycolysis fuels endothelial activation, migration and contraction and that sites of high lactate production colocalize with active cytoskeletal remodelling within an endothelial cell. Mechanistically, RhoA induces endothelial glycolysis for the phosphorylation of cofilin and myosin light chain in order to reorganize the cytoskeleton and thus control cell motility; RhoA activation triggers a glycolytic burst through the translocation of the glucose transporter SLC2A3/GLUT3 to fuel the cellular contractile machinery, as demonstrated across multiple endothelial cell types. Our data indicate that Rho-GTPase signalling coordinates energy metabolism with cytoskeleton remodelling to regulate endothelial cell motility.
|Appears in Collections:||醫學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.