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  1. NTU Scholars
  2. 醫學院
  3. 醫學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/592632
DC FieldValueLanguage
dc.contributor.authorWu, Daviden_US
dc.contributor.authorHarrison, Devin Len_US
dc.contributor.authorSzasz, Teodoraen_US
dc.contributor.authorCHIH-FAN YEHen_US
dc.contributor.authorShentu, Tzu-Pinen_US
dc.contributor.authorMeliton, Angeloen_US
dc.contributor.authorHuang, Ru-Tingen_US
dc.contributor.authorZhou, Zhengjieen_US
dc.contributor.authorMutlu, Gökhan Men_US
dc.contributor.authorHuang, Junen_US
dc.contributor.authorFang, Yunen_US
dc.date.accessioned2022-01-18T02:26:59Z-
dc.date.available2022-01-18T02:26:59Z-
dc.date.issued2021-05-
dc.identifier.issn2522-5812-
dc.identifier.urihttps://scholars.lib.ntu.edu.tw/handle/123456789/592632-
dc.description.abstractSingle-cell motility is spatially heterogeneous and driven by metabolic energy. Directly linking cell motility to cell metabolism is technically challenging but biologically important. Here, we use single-cell metabolic imaging to measure glycolysis in individual endothelial cells with genetically encoded biosensors capable of deciphering metabolic heterogeneity at subcellular resolution. We show that cellular glycolysis fuels endothelial activation, migration and contraction and that sites of high lactate production colocalize with active cytoskeletal remodelling within an endothelial cell. Mechanistically, RhoA induces endothelial glycolysis for the phosphorylation of cofilin and myosin light chain in order to reorganize the cytoskeleton and thus control cell motility; RhoA activation triggers a glycolytic burst through the translocation of the glucose transporter SLC2A3/GLUT3 to fuel the cellular contractile machinery, as demonstrated across multiple endothelial cell types. Our data indicate that Rho-GTPase signalling coordinates energy metabolism with cytoskeleton remodelling to regulate endothelial cell motility.en_US
dc.language.isoenen_US
dc.publisherNATURE RESEARCHen_US
dc.relation.ispartofNature metabolismen_US
dc.subjectRESONANCE ENERGY-TRANSFER; RHO KINASE; THROMBIN; PROLIFERATION; TRANSLOCATION; ACTIVATION; EXPRESSION; MIGRATION; DYNAMICSen_US
dc.titleSingle-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cellsen_US
dc.typejournal articleen_US
dc.identifier.doi10.1038/s42255-021-00390-y-
dc.identifier.pmid34031595-
dc.identifier.scopus2-s2.0-85106597839-
dc.identifier.isiWOS:000656451600013-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85106597839-
dc.relation.pages714en_US
dc.relation.journalvolume3en_US
dc.relation.journalissue5en_US
dc.relation.pageend727en_US
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.languageiso639-1en-
item.openairetypejournal article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
crisitem.author.deptInternal Medicine-
crisitem.author.orcid0000-0002-0678-2264-
crisitem.author.parentorgCollege of Medicine-
Appears in Collections:醫學系
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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

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