https://scholars.lib.ntu.edu.tw/handle/123456789/593534
標題: | Asialo GM1-positive liver-resident CD8 T cells that express CD44 and LFA-1 are essential for immune clearance of hepatitis B virus | 作者: | Sung, Chi-Chang Horng, Jau-Hau Siao, Shih-Hong Chyuan, I-Tsu Tsai, Hwei-Fang PEI-JER CHEN PING-NING HSU |
關鍵字: | HBV clearance; asialo GM-1; liver-resident CD8 T cells | 公開日期: | 七月-2021 | 出版社: | CHIN SOCIETY IMMUNOLOGY | 卷: | 18 | 期: | 7 | 起(迄)頁: | 1772 | 來源出版物: | Cellular & molecular immunology | 摘要: | Persistent hepatitis B virus (HBV) infection results in chronic liver diseases that may progress to chronic hepatitis, liver cirrhosis, and subsequent hepatocellular carcinoma. Previous studies demonstrated that adaptive immunity, in particular CD8 T cells, is critical in HBV elimination. Recent studies have revealed a distinct tissue-localized T cell lineage, tissue-resident memory (TRM) cells, that is crucial for protective immunity in peripheral tissues. In this study, we showed that treatment with an anti-asialo GM1 (ASGM1) antibody (Ab), which depletes NK cells, led to impairment of HBV clearance in a mouse animal model. Unexpectedly, the ability to clear HBV was not significantly impaired in NFIL3 KO mice, which are deficient in NK cells, implying that other non-NK ASGM1-positive immune cells mediate HBV clearance. We isolated intrahepatic ASGM1-positive cells from NFIL3 KO mice and analyzed the immune phenotype of these cells. Our results demonstrated a distinct population of CD44+ LFA-1hi CD8 T cells that were the major intrahepatic ASGM1-positive immune cells in NFIL3 KO mice. Importantly, transcriptome analysis revealed that these ASGM1-positive CD8 T cells had distinct gene profiles and shared a similar core gene signature with TRM cells. In addition to both transcriptional and phenotypic liver residency characteristics, ASGM1-positive CD8 T cells were able to home to and be retained in the liver after adoptive transfer. Taken together, our study results indicate that these ASGM1-positive liver-resident CD8 T cells are the major effector immune cells mediating anti-HBV immunity. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/593534 | ISSN: | 1672-7681 | DOI: | 10.1038/s41423-020-0376-0 | SDG/關鍵字: | antibody; gangliotetraosylceramide; Hermes antigen; interleukin 3; lymphocyte function associated antigen 1; nuclear factor interleukin regulated 3; transcriptome; unclassified drug; adoptive transfer; animal cell; animal experiment; animal model; Article |
顯示於: | 醫學系 |
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