https://scholars.lib.ntu.edu.tw/handle/123456789/594020
標題: | A triterpenoid-enriched extract of bitter melon leaves alleviates hepatic fibrosis by inhibiting inflammatory responses in carbon tetrachloride-treated mice | 作者: | Chang, Mei-Ling Lin, Yu-Ting HSIU-NI KUNG Hou, Yu-Chen Liu, Jun-Jen Pan, Min-Hsiung Chen, Hui-Ling Yu, Chun-Hsien MIN-HSIUNG PAN |
關鍵字: | LIVER-INJURY; MOMORDICA-CHARANTIA; FATTY LIVER; RECRUITMENT | 公開日期: | 7-九月-2021 | 出版社: | ROYAL SOC CHEMISTRY | 卷: | 12 | 期: | 17 | 起(迄)頁: | 7805 | 來源出版物: | Food & function | 摘要: | Liver fibrosis is a progression of chronic liver disease characterized by excess deposition of fibrillary collagen. The aim of this study was to investigate the protective effect of a triterpenoid-enriched extract (TEE) from bitter melon leaves against carbon tetrachloride (CCl4)-induced hepatic fibrosis in mice. Male ICR mice received TEE (100 or 150 mg kg-1) by daily oral gavage for one week before starting CCl4 administration and throughout the entire experimental period. After intraperitoneal injection of CCl4 for nine weeks, serum and liver tissues of the mice were collected for biochemical, histopathological and molecular analyses. Our results showed that TEE supplementation reduced CCl4-induced serum aspartate aminotransferase and alanine aminotransferase activities. Histopathological examinations revealed that CCl4 administration results in hepatic fibrosis, while TEE supplementation significantly suppressed hepatic necroinflammation and collagen deposition. In addition, TEE supplementation decreased α-smooth muscle actin (α-SMA)-positive staining and protein levels of α-SMA and transforming growth factor-β1. TEE-supplemented mice had lower mRNA expression levels of interleukin-6, tumor necrosis factor-α, and toll-like receptor 4. Moreover, TEE (150 mg kg-1) supplementation significantly reduced intrahepatic inflammatory Ly6C+ monocyte infiltration. We demonstrated that TEE could ameliorate hepatic fibrosis by regulating inflammatory cytokine secretion and α-SMA expression in the liver to reduce collagen accumulation. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/594020 | ISSN: | 20426496 | DOI: | 10.1039/d1fo00884f | SDG/關鍵字: | Amino acids; Cell death; Chlorine compounds; Collagen; Deposition; Mammals; Muscle; Alanine aminotransferase; Aspartate aminotransferase; Histopathological examinations; Inflammatory response; Intraperitoneal injections; MRNA expression level; Transformin |
顯示於: | 解剖學暨細胞生物學科所 |
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