https://scholars.lib.ntu.edu.tw/handle/123456789/594429
標題: | Single-cell RNA sequencing uncovers the individual alteration of intestinal mucosal immunocytes in Dusp6 knockout mice | 作者: | Chang, Cherng Shyang Yu, Wen Hsuan Su, Chang Chao Ruan, Jhen Wei Lin, Chiao Mei Huang, Chih Ting Tsai, Yi Ting Lin, I. Jung Lai, Chao Yang Chuang, Tsung Hsien Lin, Wen Jye Tan, Tse Hua Huang, Hsuan Cheng HSUEH-FEN JUAN Kao, Cheng Yuan |
關鍵字: | Biological sciences | Immunology | Transcriptomics | 公開日期: | 18-二月-2022 | 出版社: | Elsevier Inc. | 卷: | 25 | 期: | 2 | 起(迄)頁: | 103738 | 來源出版物: | iScience | 摘要: | Single-cell RNA sequencing (scRNA-seq) approach can broadly and specifically evaluate the individual cells with minimum detection bias. To explore the individual compositional and transcriptional alteration of intestinal leukocytes in the Dual Specificity Phosphatase six knockout (D6KO) mice, we performed a scRNA-seq followed by the cell type annotation based on ImmGen database. Composition assessments found that D6KO-derived intestinal leukocytes tend to stay inactivate or immature status. The enrichment analysis showed that D6KO-derived intestinal leukocytes are less sensitive to microbes. The mod PhEA phenotypic analysis showed that the D6KO leukocytes may link to not only immune-associated but also diverse previously non-immune-related diseases. Integrating our dataset with the published dataset GSE124880 generated a comprehensive dataset for exploring intestinal immunity. Down-regulation of Ccl17 gene was found in the D6KO-derived dendritic cells. Our results demonstrated the advantage of applying scRNA-seq for dissecting the individual alteration of intestinal leukocytes, particularly in the D6KO mice at a naive state. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/594429 | ISSN: | 25890042 | DOI: | 10.1016/j.isci.2022.103738 |
顯示於: | 分子與細胞生物學研究所 |
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