https://scholars.lib.ntu.edu.tw/handle/123456789/596167
標題: | Ex vivo imaging and quantification of liver fibrosis using second-harmonic generation microscopy | 作者: | Sun, T.L. Lee, Hsuan-Shu et al. Sung, M.-C. Chen, H.-C. Yang, C.H. Hovhannisyan, V. WEI-CHOU LIN YUNG-MING JENG Chen, W.-L. Chiou, L.-L. GUAN-TARN HUANG Kim, K.-H. So, P.T.C. Chen, Y.-F. Lee, H.-S. CHEN-YUAN DONG |
公開日期: | 2010 | 卷: | 15 | 期: | 3 | 起(迄)頁: | 36002 | 來源出版物: | Journal of Biomedical Optics | 摘要: | Conventionally, liver fibrosis is diagnosed using histopathological techniques. The traditional method is time-consuming in that the specimen preparation procedure requires sample fixation, slicing, and labeling. Our goal is to apply multiphoton microscopy to efficiently image and quantitatively analyze liver fibrosis specimens bypassing steps required in histological preparation. In this work, the combined imaging modality of multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) was used for the qualitative imaging of liver fibrosis of different METAVIR grades under label-free, ex vivo conditions. We found that while MAF is effective in identifying cellular architecture in the liver specimens, it is the spectrally distinct SHG signal that allows the characterization of the extent of fibrosis. We found that qualitative SHG imaging can be used for the effective identification of the associated features of liver fibrosis specimens graded METAVIR 0 to 4. In addition, we attempted to associate quantitative SHG signal to the different METAVIR grades and found that an objective determination of the extent of disease progression can be made. Our approach demonstrates the potential of using multiphoton imaging in rapid classification of ex vivo liver fibrosis in the clinical setting and investigation of liver fibrosis-associated physiopathology in animal models in vivo. ? 2010 Society of Photo-Optical Instrumentation Engineers. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-78649290222&doi=10.1117%2f1.3427146&partnerID=40&md5=12b06fa44355f8dbf2fa64761339d833 https://scholars.lib.ntu.edu.tw/handle/123456789/596167 |
ISSN: | 1083-3668 | DOI: | 10.1117/1.3427146 | SDG/關鍵字: | Animal model; Associated feature; Autofluorescence; Cellular architecture; Clinical settings; Combined imaging; Disease progression; Ex vivo imaging; Ex-vivo; In-vivo; Label free; Liver fibrosis; Liver specimens; METAVIR; Multi-photon microscopy; Multiphoton imaging; Multiphotons; Physiopathology; Preparation procedures; Qualitative imaging; SHG signals; Harmonic analysis; Specimen preparation; Harmonic generation; Animalia; acoustic microscopy; analysis of variance; article; disease course; human; immunohistochemistry; liver cirrhosis; methodology; multiphoton microscopy; pathology; signal processing; Analysis of Variance; Disease Progression; Humans; Immunohistochemistry; Liver Cirrhosis; Microscopy, Acoustic; Microscopy, Fluorescence, Multiphoton; Signal Processing, Computer-Assisted |
顯示於: | 病理學科所 |
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