https://scholars.lib.ntu.edu.tw/handle/123456789/596363
標題: | A novel peptide specifically binding to nasopharyngeal carcinoma for targeted drug delivery | 作者: | Lee T.-Y. Wu H.-C. Tseng Y.-L. CHIN-TARNG LIN |
公開日期: | 2004 | 卷: | 64 | 期: | 21 | 起(迄)頁: | 8002-8008 | 來源出版物: | Cancer Research | 摘要: | Nasopharyngeal carcinoma (NPC) is a common cancer among Chinese living in southern China, Taiwan, and Singapore. The 5-year survival rate in the early stage of NPC has been reported as high as 90 to 95% with the use of radiotherapy, but in the advanced cases, even with the use of both chemotherapy and radiotherapy, the survival rate is still <50%. To improve the survival rate, we identify a 12-mer peptide (L-peptide) specifically binding to NPC cells with a phage displayed random peptide library. The L-phage and synthetic L-peptide bound to the tumor cell surfaces of most NPC cell lines and biopsy specimens, but not normal nasal mucosal cells, and the L-peptide-linked liposomes containing fluorescent substance (L-peptide-Lipo-HPTS) were capable of binding to and translocating across plasma membranes. L-Peptide-linked liposomes that carried doxorubicin (L-peptide-Lipo-Box) caused marked cytotoxicity in NPC cells. In SCID mice bearing NPC xenografts, the L-phages specifically bound to the tumor mass, an effect that was inhibited by competition with synthetic L-peptide. In addition, the L-peptide-Lipo-Dox suppressed tumor growth better than Lipo-Dox. These results indicate that the novel L-peptide specifically binds NPC cells and is a good candidate for targeted drug delivery to NPC solid tumors. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-7444237710&doi=10.1158%2f0008-5472.CAN-04-1948&partnerID=40&md5=68d758f316e00b9562a8ee9f4059e29d https://scholars.lib.ntu.edu.tw/handle/123456789/596363 |
ISSN: | 0008-5472 | DOI: | 10.1158/0008-5472.CAN-04-1948 | SDG/關鍵字: | arginyleucylleucylaspartylthreonylasparaginylarginylprolylleucylleucyl prolyltyrosine; cholesterol; distearoylphosphatidylcholine; doxorubicin; drug carrier; liposome; peptide library; polyethylene glycol distearoylphosphatidylcholine; pyranine; pyrene derivative; synthetic peptide; unclassified drug; animal model; animal tissue; article; cancer cell culture; carcinoma cell; cell membrane; cell surface; controlled study; cytotoxicity; dose response; drug delivery system; drug targeting; female; human; human cell; human tissue; mouse; mucosa cell; nasopharynx carcinoma; nonhuman; nose mucosa; phage display; priority journal; tumor biopsy; tumor growth; tumor xenograft; Animals; Binding Sites; Doxorubicin; Endocytosis; Liposomes; Mice; Mice, SCID; Nasopharyngeal Neoplasms; Neoplasm Transplantation; Peptide Library; Peptides; Transplantation, Heterologous |
顯示於: | 病理學科所 |
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