https://scholars.lib.ntu.edu.tw/handle/123456789/596374
標題: | Response of nasopharyngeal carcinoma cells to Epstein-Barr virus infection in vitro | 作者: | CHIN-TARNG LIN Kao H.-J. Lin J.-L. Chan W.-Y. Wu H.-C. Liang S.-T. |
公開日期: | 2000 | 出版社: | Lippincott Williams and Wilkins | 卷: | 80 | 期: | 8 | 起(迄)頁: | 1149-1160 | 來源出版物: | Laboratory Investigation | 摘要: | Many nasopharyngeal carcinoma (NPC) biopsy specimens contain Epstein-Barr virus (EBV). However, the response of NPC cells to EBV infection in vitro and in vivo is not well characterized. In this experiment we infected NPC cells with EBV particles through endocytosis of a complex of EBV immunoglobulin A (IgA) secretory component (SC) protein to observe the response of host cells to the foreign viral infection in vitro. We found that EBV particles were endocytosed and stabilized in NPC nuclei 24 hours after infection; the EBV genomes were then gradually decreased after serial passages within 3 to 4 weeks by the following pathway: the EBV genomes first moved toward the nuclear envelope from the center of the nucleus; after crossing the nuclear envelope, they moved into the cytoplasm and toward the plasma membrane and were discharged by exocytosis. At the 10th day of EBV infection, EBV-latent membrane protein-1 and Epstein-Barr nuclear antigen (EBNA)-1 protein expressions could be detected, but not EBV-viral capsid antigen. Observation of EBNA-1 protein and host growth factor and cytokine gene expressions in the weeks after incubation revealed that the EBNA-1 protein expression was decreased proportionally with decrease of EBV genome. The mRNA expression of epithelial growth factor receptor, transforming growth factor (TGF)-α, interleukin (IL)-1β, IL-6, and granulocyte-macrophage colony-stimulating factor increased within 1 to 2 weeks after infection, and gradually recovered to the original level at 3 to 4 weeks, whereas the mRNAs of TGFβ1, TGFβ receptor type I (TGFβRI), TGFβR type II, IL-8, and tumor necrosis factor-α remained unchanged. It is concluded that in vitro EBV infection in NPC cells results in increase of certain growth factor and cytokine gene expressions in host cells. The change in gene expression returns to the original level approximately 3 to 4 weeks after infection because of exocytosis of EBV DNA by the infected cells through an unidentified mechanism. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033870236&doi=10.1038%2flabinvest.3780123&partnerID=40&md5=896a46b2605f81aec26d5c292dc82dec https://scholars.lib.ntu.edu.tw/handle/123456789/596374 |
ISSN: | 0023-6837 | DOI: | 10.1038/labinvest.3780123 | SDG/關鍵字: | cell nucleus antigen; granulocyte macrophage colony stimulating factor; growth factor receptor; immunoglobulin A; interleukin 1beta; interleukin 6; interleukin 8; latent membrane protein 1; secretory component; transforming growth factor alpha; transforming growth factor beta receptor; transforming growth factor beta1; tumor necrosis factor; virus capsid antigen; article; cancer cell; cell nucleus membrane; controlled study; endocytosis; Epstein Barr virus; exocytosis; gene expression; genome; human; human cell; immune response; nasopharynx carcinoma; priority journal; protein expression; sequence analysis; virus infection |
顯示於: | 病理學科所 |
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