https://scholars.lib.ntu.edu.tw/handle/123456789/596725
標題: | Production of monoclonal antibody for okadaic acid and its utilization in an ultrasensitive enzyme-linked immunosorbent assay and one-step immunochromatographic strip | 作者: | Liu, Biing-Hui Hung C.-T. Lu C.-C. Chou H.-N. Yu F.-Y. |
關鍵字: | enzyme-linked immunosorbent assay; gold nanoparticle immunochromatographic strip; monoclonal antibody; okadaic acid | 公開日期: | 2014 | 卷: | 62 | 期: | 6 | 起(迄)頁: | 1254-1260 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | Okadaic acid (OA) is a common marine biotoxin that accumulates in bivalves and causes diarrhetic shellfish poisoning (DSP). This study generated a monoclonal antibody (mAb) specific to OA from a hybridoma cell line, 6B1A3, which was obtained by fusion of myeloma cells (P3/NS1/1-AG4-1) with spleen cells isolated from a BALB/c mouse immunized with OA-γ-globulin. The 6B1A3 mAb belongs to the immunoglobulin G1 (κ chain) isotype. Both competitive direct and indirect enzyme-linked immunosorbent assays (ELISAs) were established for characterization of the antibody. The concentrations causing 50% inhibition of binding of OA-horseradish peroxidase to the antibody by OA were calculated to be 0.077 ng/mL in the cdELISA. A rapid and sensitive mAb-based gold nanoparticle immunochromatographic strip was also established. This proposed strip has a detection limit of 5 ng/mL for OA and can be finished in 10 min. Extensive analyses of 20 seafood samples with ELISA revealed that 10 were slightly contaminated with OA, with a mean concentration of 0.892 ng/g. Analysis of OA in shellfish samples showed that data acquired by the immunochromatographic strip agreed well with those acquired by the ELISA. The mAb-based ELISA and immunochromatographic strip assay developed in this study have adequate sensitivity and accuracy for rapid screening of OA in shellfish samples. © 2014 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894189227&doi=10.1021%2fjf404827s&partnerID=40&md5=413db2d150db3820151701a06dceb1a6 https://scholars.lib.ntu.edu.tw/handle/123456789/596725 |
ISSN: | 0021-8561 | DOI: | 10.1021/jf404827s | SDG/關鍵字: | monoclonal antibody; okadaic acid; animal; article; Bagg albino mouse; biosynthesis; cell line; enzyme linked immunosorbent assay; female; hybridoma; immunoaffinity chromatography; immunology; methodology; mouse; sensitivity and specificity; shellfish; shellfish poisoning; test strip; Animals; Antibodies, Monoclonal; Cell Line; Enzyme-Linked Immunosorbent Assay; Female; Hybridomas; Immunochromatography; Mice; Mice, Inbred BALB C; Okadaic Acid; Reagent Strips; Sensitivity and Specificity; Shellfish; Shellfish Poisoning; Assays; Bioassay; Cell culture; Enzymes; Gold; Metal nanoparticles; Monoclonal antibodies; Nanoparticles; Screening; Shellfish; Diarrhetic shellfish poisoning; Enzyme linked immunosorbent assay; Gold Nanoparticles; Horse-radish peroxidase; Immunochromatographic strips; Mean concentrations; Monoclonal antibodies (mAb); Okadaic acid; Antibodies; Armoracia rusticana; Bivalvia |
顯示於: | 毒理學研究所 |
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