Comparison of two-and three-dimensional nuclear to cytoplasm ratios in analyzing lung cancer cell lines
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
10881
Date Issued
2019
Author(s)
Huang H.-C.
Chiang, S.-J.
Wen, S.-H.
Lee, P.-J.
Chen, Y.-F.
Abstract
Histological examination has been the primary imaging modality in the diagnosis of eases such as cancer. However, since cells are three-dimensional in nature, the use of traditional nucleus to cytoplasm ratio (N/C) in two dimensions does not represent their three-dimensional structures. In this study, we used two-photon microscopy to acquired threedimensional images of normal human lung cell line Beas2B, human lung adenocarcinoma CL1-0 and CL1-5 cell lines. We determined N/C ratios in two- and three-dimensions and found that 2D N/C-ratio is more precise than 3D N/C-ratio in discriminating normal and cancer cells.
Publisher
SPIE
Type
conference paper