https://scholars.lib.ntu.edu.tw/handle/123456789/597077
標題: | Clonal Origin Evaluated by Trunk and Branching Drivers and Prevalence of Mutations in Multiple Lung Tumor Nodules | 作者: | Rodriguez E.F. LI-HUI TSENG De Marchi F. Haung J. Belchis D. Xian R. Gocke C.D. Eshleman J.R. Illei P.B. Lin M.-T. |
公開日期: | 2020 | 出版社: | Adis | 卷: | 24 | 期: | 4 | 起(迄)頁: | 461-472 | 來源出版物: | Molecular Diagnosis and Therapy | 摘要: | Introduction: Differentiation between intrapulmonary metastasis (IPM) and multiple primary lung cancers (MPLC) in patients with synchronous or metachronous lung tumor nodules is critical but challenging. Objective: We proposed an algorithm to evaluate clonal origin based on trunk (initiating) versus branching drivers and the prevalence of mutations in lung adenocarcinomas. Methods: Driver mutations were examined using next-generation sequencing in five trunk driver genes (BRAF, EGFR, ERBB2, KRAS, and NRAS) and three branching driver genes (ATK1, PIK3CA, and TP53). Results: Mutational profiling supported same clonality and likely same clonality, respectively, in 39 and 14 of 66 pairs of specimens with known identical clonal origin. Discordance of TP53 mutations (branching drivers) was observed in three pairs. Subsequent analyses of 30 pairs of synchronous or metachronous lung tumor nodules revealed different clonality and likely different clonality in 17 and 2 pairs, respectively, including three pairs with similar histomorphology; same clonality and likely same clonality in three and five pairs, respectively, including two pairs with different histomorphology; and inconclusive or noninformative results in three pairs. Conclusion: While discordance of trunk drivers indicated MPLC in patients with synchronous or metachronous lung tumor nodules, discordance of branching drivers did not exclude IPM. Concordance of uncommon drivers supported IPM, whereas concordance of common drivers did not exclude MPLC. Additional recommendations from official organizations are needed to guide applications of molecular markers in defining clonality of multiple lung tumor nodules. ? 2020, Springer Nature Switzerland AG. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086114181&doi=10.1007%2fs40291-020-00471-w&partnerID=40&md5=0e750ec29f9492fdcd6763f72cbecbf6 https://scholars.lib.ntu.edu.tw/handle/123456789/597077 |
ISSN: | 1177-1062 | DOI: | 10.1007/s40291-020-00471-w | SDG/關鍵字: | akt1 protein; B Raf kinase; epidermal growth factor receptor; epidermal growth factor receptor 2; K ras protein; molecular marker; pik3ca protein; protein p53; Ras protein; unclassified drug; protein p53; Article; cancer staging; cancer surgery; clonal variation; controlled study; fine needle aspiration biopsy; gene frequency; gene mutation; high throughput sequencing; human; human tissue; lung adenocarcinoma; lung biopsy; metastasis; multiple pulmonary nodules; prevalence; primary tumor; priority journal; single nucleotide polymorphism; smoking; adenocarcinoma; gene; genetics; high throughput sequencing; lung tumor; mutation; procedures; Adenocarcinoma; Genes; High-Throughput Nucleotide Sequencing; Humans; Lung Neoplasms; Mutation; Prevalence; Tumor Suppressor Protein p53 |
顯示於: | 基因體暨蛋白體醫學研究所 |
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