https://scholars.lib.ntu.edu.tw/handle/123456789/597112
標題: | Tandem duplication PCR: An ultrasensitive assay for the detection of internal tandem duplications of the FLT3 Gene | 作者: | Lin M.-T. LI-HUI TSENG Beierl K. Hsieh A. Thiess M. Chase N. Stafford A. Levis M.J. Eshleman J.R. Gocke C.D. |
關鍵字: | acute myeloid leukemia; FLT3; internal tandem duplication; minimal residual disease; tandem duplication PCR | 公開日期: | 2013 | 卷: | 22 | 期: | 3 | 起(迄)頁: | 149-155 | 來源出版物: | Diagnostic Molecular Pathology | 摘要: | Internal tandem duplication (ITD) mutations of the FLT3 gene have been associated with a poor prognosis in acute myeloid leukemia. Detection of ITD-positive minor clones at the initial diagnosis and during the minimal residual disease stage may be essential. We previously designed a delta-PCR strategy to improve the sensitivity to 0.1% ITD-positive leukemia cells and showed that minor mutants with an allele burden of <1% can be clinically significant. In this study, we report on tandem duplication PCR (TD-PCR), a modified inverse PCR assay, and demonstrate a limit of detection of a few molecules of ITD mutants. The TD-PCR was initially designed to confirm ITD mutation of an amplicon, which was undetectable by capillary electrophoresis and was incidentally isolated by a molecular fraction collecting tool. Subsequently, TD-PCR detected ITD mutation in 2 of 77 patients previously reported as negative for ITD mutation by a standard PCR assay. TD-PCR can also potentially be applied to monitor minimal residual disease with high analytic sensitivity in a portion of ITD-positive acute myeloid leukemia patients. Further studies using TD-PCR to detect ITD mutants at diagnosis may clarify the clinical significance of those ITD mutants with extremely low allele burden. Copyright ? 2013 by Lippincott Williams & Wilkins. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883465084&doi=10.1097%2fPDM.0b013e31828308a1&partnerID=40&md5=cc020c503a76f41de6a80623051344d5 https://scholars.lib.ntu.edu.tw/handle/123456789/597112 |
ISSN: | 1052-9551 | DOI: | 10.1097/PDM.0b013e31828308a1 | SDG/關鍵字: | acute granulocytic leukemia; article; cancer prognosis; controlled study; FLT3 gene; gene; gene duplication; gene mutation; genetic analysis; human; human cell; human tissue; leukemia cell; limit of detection; major clinical study; polymerase chain reaction; priority journal; tandem repeat |
顯示於: | 基因體暨蛋白體醫學研究所 |
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