Analysis of hematopoietic stem cell transplant engraftment: Use of loss or gain of microsatellite alleles to identify residual hematopoietic malignancy
Journal
Diagnostic Molecular Pathology
Journal Volume
20
Journal Issue
4
Pages
194-202
Date Issued
2011
Author(s)
Abstract
Polymorphic short tandem repeat (STR), or microsatellite, loci have been widely used to analyze chimerism status after allogeneic hematopoietic stem cell transplantation. The presence of a patient's DNA, as identified by STR analysis, may indicate residual or recurrent malignant disease or may represent normal hematopoiesis of patient origin. The ratio of patient-derived to donor-derived alleles is used to calculate the relative amount of patient cells (both benign and malignant) to donor cells. STRs on chromosomes known to be gained or lost in a patient's tumor are generally ignored because it is difficult to perform meaningful calculations of mixed chimerism. However, in this study, we present evidence that STR loci on gained or lost chromosomes are useful in distinguishing the benign or malignant nature of chimeric DNA. In the peripheral blood or bone marrow of 4 hematopoietic stem cell transplantation patients with leukemia or lymphoma, we identified tumor DNA on the basis of STR loci showing copy number alteration. We propose that a targeted evaluation of STR loci showing altered copy number in posttransplant chimerism analysis can provide evidence of residual cancer cells. ? 2011 by Lippincott Williams & Wilkins.
Subjects
allogeneic hematopoietic stem cell transplantation; chimerism; leukemia relapse; microsatellite; Short tandem repeat
SDGs
Other Subjects
acute granulocytic leukemia; adult; aged; allele; article; blood; bone marrow; cancer cell; cancer chemotherapy; cancer patient; case report; chemoradiotherapy; child; chimera; chromosome; copy number variation; engraftment; female; gene locus; hematopoietic stem cell transplantation; human; large cell lymphoma; male; monosomy 7; multiple myeloma; myelodysplastic syndrome; preschool child; priority journal; short tandem repeat; T cell lymphoma; tumor biopsy; Adult; Aged; Alleles; Child, Preschool; DNA, Neoplasm; Female; Hematopoietic Stem Cell Transplantation; Humans; Leukemia; Lymphoma; Male; Microsatellite Repeats; Middle Aged; Neoplasm, Residual
Type
journal article