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  1. NTU Scholars
  2. 醫學院
  3. 微生物學科所
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/597270
Title: Topoisomerase II-mediated DNA cleavage and mutagenesis activated by nitric oxide underlie the inflammation-associated tumorigenesis
Authors: Yang Y.-C.
Chou H.-Y.E.
Shen T.-L.
Chang W.-J.
Tai P.-H.
TSAI-KUN LI 
Issue Date: 2013
Journal Volume: 18
Journal Issue: 10
Start page/Pages: 1129-1140
Source: Antioxidants and Redox Signaling
Abstract: 
Aims: Both cancer-suppressing and cancer-promoting properties of reactive nitrogen and oxygen species (RNOS) have been suggested to play a role in tumor pathology, particularly those activities associated with chronic inflammation. Here, we address the impact of nitric oxide (NO) on the induction of DNA damage and genome instability with a specific focus on the involvement of topoisomerase II (TOP2). We also investigate the contribution of NO to the formation of skin melanoma in mice. Results: Similar to the TOP2-targeting drug, etoposide (VP-16), the NO-donor, S-nitrosoglutathione (GSNO), induces skin melanomas formation in 7,12-dimethyl-benz[a]anthracene (DMBA)-initiated mice. To explore the mechanism(s) underlying this NO-induced tumorigenesis, we use a co-culture model system to demonstrate that inflamed macrophages with inducible NO synthase (iNOS) expression cause γ-H2AX activation, p53 phosphorylation, and chromosome DNA breaks in the target cells. Inhibitor experiments revealed that NO and TOP2 isozymes are responsible for the above described cellular phenotypes. Notably, NO, unlike VP-16, preferentially induces the formation of TOP2β cleavable complexes (TOP2βcc) in cells. Moreover, GSNO induced TOP2-dependent DNA sequence rearrangements and cytotoxicity. Furthermore, the incidences of GSNO-and VP-16-induced skin melanomas were also observed to be lower in the skin-specific top2β-knockout mice. Our results suggest that TOP2 isozymes contribute to NO-induced mutagenesis and subsequent cancer development during chronic inflammation. Innovation and Conclusions: We provide the first experimental evidence for the functional role of TOP2 in NO-caused DNA damage, mutagenesis, and carcinogenesis. Notably, these studies contribute to our molecular understanding of the cancer-promoting actions of RNOS during chronic inflammation. Antioxid. Redox Signal. 18, 1129-1140. ? 2013, Mary Ann Liebert, Inc.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874059703&doi=10.1089%2fars.2012.4620&partnerID=40&md5=61e87fecef36ebea8ab17c804dba077c
https://scholars.lib.ntu.edu.tw/handle/123456789/597270
ISSN: 1523-0864
DOI: 10.1089/ars.2012.4620
metadata.dc.subject.other: dimethylbenz[a]anthracene; DNA topoisomerase (ATP hydrolysing); etoposide; histone H2AX; inducible nitric oxide synthase; nitric oxide; protein p53; s nitrosoglutathione; animal experiment; article; carcinogenesis; controlled study; cytotoxicity; DNA cleavage; DNA sequence; DNA strand breakage; enzyme phosphorylation; inflammation; macrophage; melanoma; mouse; mutagenesis; nonhuman; phenotype; priority journal; 9,10-Dimethyl-1,2-benzanthracene; Animals; Cell Line; Cell Transformation, Neoplastic; Coculture Techniques; DNA Cleavage; DNA Topoisomerases, Type II; Etoposide; HCT116 Cells; HL-60 Cells; Humans; Inflammation; Mice; Mice, Knockout; Mutagenesis; Nitric Oxide; Nitric Oxide Donors; Pyridines; S-Nitrosoglutathione
[SDGs]SDG3
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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

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