https://scholars.lib.ntu.edu.tw/handle/123456789/597464
標題: | Transcriptomic Analysis Suggests Auxin Regulation in Dorsal–Ventral Petal Asymmetry of Wild Progenitor Sinningia speciosa | 作者: | Pan, Zhao Jun Nien, Ya Chi Shih, Yu An Chen, Tsun Ying Lin, Wen Dar Kuo, Wen Hsi Hsu, Hao Chun Tu, Shih Long JEN-CHIH CHEN |
關鍵字: | Auxin | Cell growth | Floral symmetry | Petal pigmentation | Petal size | 公開日期: | 1-二月-2022 | 出版社: | MDPI | 卷: | 23 | 期: | 4 | 起(迄)頁: | Article number 2073 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | The establishment of dorsal–ventral (DV) petal asymmetry is accompanied by differential growth of DV petal size, shape, and color differences, which enhance ornamental values. Genes involved in flower symmetry in Sinningia speciosa have been identified as CYCLOIDEA (SsCYC), but which gene regulatory network (GRN) is associated with SsCYC to establish DV petal asymmetry is still unknown. To uncover the GRN of DV petal asymmetry, we identified 630 DV differentially expressed genes (DV-DEGs) from the RNA-Seq of dorsal and ventral petals in the wild progenitor, S. speciosa ‘ES’. Validated by qRT-PCR, genes in the auxin signaling transduction pathway, SsCYC, and a major regulator of anthocyanin biosynthesis were upregulated in dorsal petals. These genes correlated with a higher endogenous auxin level in dorsal petals, with longer tube length growth through cell expansion and a purple dorsal color. Over-expression of SsCYC in Nicotiana reduced petal size by regulating cell growth, suggesting that SsCYC also controls cell expansion. This suggests that auxin and SsCYC both regulate DV petal asymmetry. Transiently over-expressed SsCYC, however, could not activate most major auxin signaling genes, suggesting that SsCYC may not trigger auxin regulation. Whether auxin can activate SsCYC or whether they act independently to regulate DV petal asymmetry remains to be explored in the future. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/597464 | ISSN: | 16616596 | DOI: | 10.3390/ijms23042073 |
顯示於: | 生命科學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。