https://scholars.lib.ntu.edu.tw/handle/123456789/597479
標題: | Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in Arabidopsis | 作者: | Hu, Shu Hsuan TSUNG-LUO JINN |
關鍵字: | Fe/Mn ratio | Mitochondrial carrier protein | Mn transporter | MnSOD | SOD | Superoxide | 公開日期: | 1-三月-2022 | 出版社: | MDPI | 卷: | 11 | 期: | 5 | 起(迄)頁: | Article number 619 | 來源出版物: | Plants | 摘要: | It has been reported that the mitochondrial carrier family proteins of AtMTM1 and AtMTM2 are necessary for manganese superoxide dismutase (MnSOD) activation in Arabidopsis, and are responsive to methyl viologen (MV)-induced oxidative stress. In this study, we showed that MnSOD activity was enhanced specifically by Mn treatments. By using AtMnSOD-overexpressing and AtMnSOD-knockdown mutant plants treated with the widely used oxidative stressors including MV, NaCl, H2 O2, and tert-butyl hydroperoxide (t-BH), we revealed that Arabidopsis MnSOD was crucial for root-growth control and superoxide scavenging ability. In addition, it has been reported that E. coli MnSOD activity is inhibited by Fe and that MTM1-mutated yeast cells exhibit elevated Fe content and decreased MnSOD activity, which can be restored by the Fe2+-specific chelator, bathophenanthroline disulfonate (BPS). However, we showed that BPS inhibited MnSOD activity in AtMTM1 and AtMTM2 single-and double-mutant protoplasts, implying that altered Fe homeostasis affected MnSOD activation through AtMTM1 and AtMTM2. Notably, we used inductively coupled plasma-optical emission spectrometry (ICP-OES) analysis to reveal an abnormal Fe/Mn ratio in the roots and shoots of AtMTM1 and AtMTM2 mutants under MV stress, indicating the importance of AtMTM1 in roots and AtMTM2 in shoots for maintaining Fe/Mn balance. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/597479 | ISSN: | 22237747 | DOI: | 10.3390/plants11050619 |
顯示於: | 植物科學研究所 |
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